PMID- 192340 OWN - NLM STAT- MEDLINE DCOM- 19770611 LR - 20070723 IS - 0320-9725 (Print) IS - 0320-9725 (Linking) VI - 41 IP - 8 DP - 1976 Aug TI - [On hexokinase isozymes]. PG - 1504-9 AB - Electrophoretic study of hexokinase (HK) associated with the soluble fraction of mouse transplantable hepatoma 22a revealed that almost all bands of HK activities overlapped the bands of glucose-6-P dehydrogenase (G6PDH) activities in the gels. Similar results were obtained for liver, muscle and brain soluble fractions, as well as for various extracts from hepatoma 22a mitochondria and commercial preparation of yeast HK. A single type of HK, which does not overlap G6PDH activity, was located between types I and II (according to the Katzen classification) as a diffuse band of 1 hour manifestation. A possibility of structural organization of glycolytic enzymes in the cell essential for the quantitative estimation of the isozyme pattern is discussed. FAU - Gobeev, V N AU - Gobeev VN FAU - Khripach, L V AU - Khripach LV LA - rus PT - English Abstract PT - Journal Article TT - K voprosu ob izozimakh geksokinazy. PL - Russia (Federation) TA - Biokhimiia JT - Biokhimiia (Moscow, Russia) JID - 0372667 RN - 0 (Isoenzymes) RN - EC 1.1.1.49 (Glucosephosphate Dehydrogenase) RN - EC 2.7.1.1 (Hexokinase) SB - IM MH - Animals MH - Brain/metabolism MH - Carcinoma, Hepatocellular/*enzymology MH - Glucosephosphate Dehydrogenase MH - *Hexokinase MH - *Isoenzymes MH - Liver/enzymology MH - Liver Neoplasms/*enzymology MH - Mice MH - Mitochondria, Liver/enzymology MH - Muscles/enzymology MH - Neoplasms, Experimental/enzymology MH - Solubility MH - Subcellular Fractions/enzymology EDAT- 1976/08/01 00:00 MHDA- 1976/08/01 00:01 CRDT- 1976/08/01 00:00 PHST- 1976/08/01 00:00 [pubmed] PHST- 1976/08/01 00:01 [medline] PHST- 1976/08/01 00:00 [entrez] PST - ppublish SO - Biokhimiia. 1976 Aug;41(8):1504-9.