PMID- 19235261
OWN - NLM
STAT- MEDLINE
DCOM- 20090602
LR  - 20211020
IS  - 1522-2594 (Electronic)
IS  - 0740-3194 (Print)
IS  - 0740-3194 (Linking)
VI  - 61
IP  - 3
DP  - 2009 Mar
TI  - Evaluation of the ERETIC method as an improved quantitative reference for 1H 
      HR-MAS spectroscopy of prostate tissue.
PG  - 525-32
LID - 10.1002/mrm.21808 [doi]
AB  - The Electronic REference To access In vivo Concentrations (ERETIC) method was 
      applied to (1)H HR-MAS spectroscopy. The accuracy, precision, and stability of 
      ERETIC as a quantitative reference were evaluated in solution and human prostate 
      tissue samples. For comparison, the reliability of 
      3-(trimethylsilyl)propionic-2,2,3,3-d(4) acid (TSP) as a quantitation reference 
      was also evaluated. The ERETIC and TSP peak areas were found to be stable in 
      solution over the short-term and long-term, with long-term relative standard 
      deviations (RSDs) of 4.10% and 2.60%, respectively. Quantification of TSP in 
      solution using the ERETIC peak as a reference and a calibrated, rotor-dependent 
      conversion factor yielded results with a precision < or =2.9% and an accuracy 
      error < or =4.2% when compared with the expected values. The ERETIC peak area 
      reproducibility was superior to TSP's reproducibility, corrected for mass, in 
      both prostate surgical and biopsy samples (4.53% vs. 21.2% and 3.34% vs. 31.8%, 
      respectively). Furthermore, the tissue TSP peaks exhibited only 27.5% of the 
      expected area, which would cause an overestimation of metabolite concentrations 
      if used as a reference. The improved quantification accuracy and precision 
      provided by ERETIC may enable the detection of smaller metabolic differences that 
      may exist between individual tissue samples and disease states.
FAU - Albers, Mark J
AU  - Albers MJ
AD  - Department of Bioengineering, University of California Berkeley, USA.
FAU - Butler, Thomas N
AU  - Butler TN
FAU - Rahwa, Iman
AU  - Rahwa I
FAU - Bao, Nguyen
AU  - Bao N
FAU - Keshari, Kayvan R
AU  - Keshari KR
FAU - Swanson, Mark G
AU  - Swanson MG
FAU - Kurhanewicz, John
AU  - Kurhanewicz J
LA  - eng
GR  - K01 CA096618/CA/NCI NIH HHS/United States
GR  - R01 CA102751/CA/NCI NIH HHS/United States
GR  - R01 CA102751-01A2/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Magn Reson Med
JT  - Magnetic resonance in medicine
JID - 8505245
RN  - 0 (Protons)
SB  - IM
MH  - *Algorithms
MH  - Humans
MH  - Magnetic Resonance Spectroscopy/*methods/*standards
MH  - Male
MH  - Prostate/*metabolism
MH  - Protons
MH  - Reference Values
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - United States
PMC - PMC2879886
MID - NIHMS192364
EDAT- 2009/02/24 09:00
MHDA- 2009/06/03 09:00
PMCR- 2010/06/02
CRDT- 2009/02/24 09:00
PHST- 2009/02/24 09:00 [entrez]
PHST- 2009/02/24 09:00 [pubmed]
PHST- 2009/06/03 09:00 [medline]
PHST- 2010/06/02 00:00 [pmc-release]
AID - 10.1002/mrm.21808 [doi]
PST - ppublish
SO  - Magn Reson Med. 2009 Mar;61(3):525-32. doi: 10.1002/mrm.21808.