PMID- 19236738 OWN - NLM STAT- MEDLINE DCOM- 20110823 LR - 20161018 IS - 1009-2137 (Print) IS - 1009-2137 (Linking) VI - 17 IP - 1 DP - 2009 Feb TI - [MICM characteristics and typing diagnosis in acute myelogenous leukemia patients (AML-M2) with complex karyotype t (2;21;8)(p12;q22;q22)]. PG - 12-6 AB - This study was purposed to investigate the acute myeloid leukemia with complex karyotype t(2;21;8)(p12;q22;q22) (AML-M(2)) by using morphologic, immunologic, cytogenetic and molecular biologic classification technique (MICM) and to analyze the MICM characteristics of AML-M(2) and their diagnostic significance. The FAB typing of bone marrow cells (BMCs) was performed by Wright-Giemsa staining and histochemical staining of BM smears; the immunophenotype of leukemic cells was detected by flow cytometry; the karyotypes of chromosome samples prepared by short-term (48 hours) conventional culture of fresh BMCs were analyzed by RHG banding technique; the FISH signaling in mitotic metaphase was determined by dual color and dual fusion AML/ETO probe and chromosome painting probe, and was compared with results of conventional cytogenetic assay; the AML/ETO fusion transcripts were detected by nested RT-PCR. The results indicated that the bone marrow smears of case 1 showed extremely hyperplasia with myeloblasts in which a ratio of eosinophilic granulocytes and monocytes increased. Case 2 accorded with AML-M(2b) in which abnormal increase of myelocytes mainly appeared. The complex karyotype t(2;21;8)(p12;q22;q22) was detected by cytogenetic analysis combined with FISH in both two cases and AML1/ETO fusion transcripts were found by RT-PCR as well. The immunophenotype assay showed high co-expression of CD34 and HLA-DR accompanied with CD19 and CD56 expressions. It is concluded that application of MICM has an important significance for correct diagnostic typing of AML-M2 with complex karyotype variant of t(8; 21)(p12;q22;q22). FAU - Ma, Yu AU - Ma Y AD - Center of Hematologic Malignancies, China Medical University, Shenyang 110022, China. FAU - Tong, Hai-Xia AU - Tong HX FAU - Deng, Xin AU - Deng X FAU - Zhao, Yi AU - Zhao Y FAU - Liu, Zhuo-Gang AU - Liu ZG FAU - Zhang, Ji-Hong AU - Zhang JH LA - chi PT - Case Reports PT - English Abstract PT - Journal Article PL - China TA - Zhongguo Shi Yan Xue Ye Xue Za Zhi JT - Zhongguo shi yan xue ye xue za zhi JID - 101084424 SB - IM MH - Adult MH - Chromosomes, Human, Pair 2 MH - Chromosomes, Human, Pair 21 MH - Chromosomes, Human, Pair 8 MH - Female MH - Humans MH - Immunophenotyping MH - Karyotyping MH - Leukemia, Myeloid, Acute/*diagnosis/*genetics/immunology MH - Male MH - Middle Aged EDAT- 2009/02/25 09:00 MHDA- 2011/08/24 06:00 CRDT- 2009/02/25 09:00 PHST- 2009/02/25 09:00 [entrez] PHST- 2009/02/25 09:00 [pubmed] PHST- 2011/08/24 06:00 [medline] AID - 1009-2137(2009)01-0012-05 [pii] PST - ppublish SO - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Feb;17(1):12-6.