PMID- 19236742
OWN - NLM
STAT- MEDLINE
DCOM- 20110823
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 17
IP  - 1
DP  - 2009 Feb
TI  - [Detection of molecular cytogenetic abnormalities in 30 patients with chronic 
      lymphocytic leukemia by fluorescence in situ hybridization].
PG  - 31-5
AB  - This study was aimed to investigate the significance of interphase fluorescence 
      in situ hybridization (FISH) in detecting +12, del (13q14), p53 and atm gene 
      deletion in chronic lymphocytic leukemia (CLL). FISH and a panel of probes (CEP 
      12, LSI D13S319, LSI p53, LSI atm) were used to detect molecular cytogenetic 
      abnormalities in 30 patients with CLL. Cytogenetic aberrations and their relation 
      with some other prognostic factors (peripheral lymphocyte count, Binet stage, LDH 
      level, ZAP-70 and so on) were analyzed. The results indicated that out of the 30 
      CLL patients, molecular cytogenetic aberrations were found in 19 (63.3%) cases 
      and 7 (23.3%) patients showed more than two kinds of abnormalities. The most 
      frequent abnormality detected was del (13q14) (43.3%), followed by trisomy of 
      chromosome 12 (23.3%), del (atm) (13.3%) and del (p53) (10.0%). There were no 
      significant differences between molecular cytogenetic aberrations and sex, age, 
      Binet stage, peripheral lymphocyte count, or the serum levels of lactate 
      dehydrogenase (LDH), beta(2)-microglobulin (beta(2)-MG), or ZAP-70. The incidence 
      of atm gene deletion was higher in the group of CD38 high expression than that in 
      the group of low expression (p = 0.035). It is concluded that FISH is a rapid and 
      sensitive technique in analysing molecular cytogenetic abnormalities, but its 
      prognostic significance in CLL needs to further investigate.
FAU - Dai, Dan
AU  - Dai D
AD  - Department of Hematology, The First Affilated Hospital, Nanjing Medical 
      University, Nanjing 210006, China.
FAU - Zhang, Xiu-Qun
AU  - Zhang XQ
FAU - Zhang, Xue-Zhong
AU  - Zhang XZ
FAU - Su, Ai-Ling
AU  - Su AL
FAU - Zhang, Lei
AU  - Zhang L
FAU - Cao, Shi-Bin
AU  - Cao SB
FAU - Xu, Yan-Li
AU  - Xu YL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Female
MH  - Gene Deletion
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
EDAT- 2009/02/25 09:00
MHDA- 2011/08/24 06:00
CRDT- 2009/02/25 09:00
PHST- 2009/02/25 09:00 [entrez]
PHST- 2009/02/25 09:00 [pubmed]
PHST- 2011/08/24 06:00 [medline]
AID - 1009-2137(2009)01-0031-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Feb;17(1):31-5.