PMID- 19236745
OWN - NLM
STAT- MEDLINE
DCOM- 20110823
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 17
IP  - 1
DP  - 2009 Feb
TI  - [Inhibition effect of diimide G-quadruplex ligand on proliferation of leukemia 
      cells and its molecular mechanisms].
PG  - 43-8
AB  - This study was aimed to investigate the growth inhibition effect of diimide 
      G-quadruplex ligand on leukemia cells and to explore its molecular mechanisms. 
      K562 leukemia cell lines were treated with various concentrations of the diimide 
      G-quadruples ligand small molecule (0.1 - 10 micromol/L). Trypan blue exclusion 
      assay was used to evaluate the proliferation inhibition. Cell apoptosis was 
      observed using terminal deoxynucleotide transferase-mediated dUTP nick end 
      labeling (TUNEL). Telomerase activity was analyzed by telomere repeat 
      amplification protocol. Gene expression was detected by microarray and confirmed 
      by RT-PCR assay. The results showed that diimide small molecule inhibited the 
      proliferation of K562 cells and induced apoptosis of these cells. After treating 
      with diimide G-quadruplex ligand, telomerase activity of K562 cells was reduced 
      and the transcriptional levels of some important genes were changed 
      significantly. These genes were involved in cell apoptosis, cell signaling 
      pathway and other key functions. In conclusion, the diimide G-quadruplex ligand 
      is a small molecule that inhibits the proliferation and induces apoptosis in 
      leukemia cells, and these functions may be related to telomerase inhibition and 
      regulation of some important gene transcription.
FAU - Chu, Bin
AU  - Chu B
AD  - Laboratory of Hematology, Peking University First Hospital, Beijing 100034, 
      China.
FAU - Yuan, Gu
AU  - Yuan G
FAU - Zhou, Jiang
AU  - Zhou J
FAU - Ou, Yuan
AU  - Ou Y
FAU - Zhu, Ping
AU  - Zhu P
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Ligands)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Proliferation/*drug effects
MH  - *G-Quadruplexes
MH  - Humans
MH  - K562 Cells
MH  - Leukemia/*genetics/*pathology
MH  - Ligands
MH  - Microarray Analysis
MH  - Telomerase/metabolism
EDAT- 2009/02/25 09:00
MHDA- 2011/08/24 06:00
CRDT- 2009/02/25 09:00
PHST- 2009/02/25 09:00 [entrez]
PHST- 2009/02/25 09:00 [pubmed]
PHST- 2011/08/24 06:00 [medline]
AID - 1009-2137(2009)01-0043-06 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Feb;17(1):43-8.