PMID- 19236764 OWN - NLM STAT- MEDLINE DCOM- 20110823 LR - 20161125 IS - 1009-2137 (Print) IS - 1009-2137 (Linking) VI - 17 IP - 1 DP - 2009 Feb TI - [Detection and analysis of CD271, CD133 and CD34 expressions in bone marrow cells by flow cytometry with three color fluorescence labelling]. PG - 133-6 AB - This study was purposed to detect the expressions of CD271, CD133 and CD34, and to analyze the correlation of CD271 with CD133 and CD133 with CD34 expressions. The human bone marrow cells (BMCs) and mononucleated cells (MNCs) were detected by flow cytometry with CD45-PerCP, CD271-FITC, CD133-PE and CD34-FITC labelling according to different combinations of design, cells were located and selected repeatedly by FSC, SSC and CD45 after acquirement, then the expressions of CD271, CD133 and CD34 were detected by flow cytometry. The results showed that the expressions of CD271, CD133 and CD34 in BMCs were 0.16%, 0.20% and 0.43% respectively, while their expressions were 0.49%, 0.47% and 1.07% respectively after isolation of MNCs. The co-expressions of CD271(+)CD133(+) before and after isolation of MNCs were (0.02 +/- 0.01)% and (0.03 +/- 0.02)% respectively. The co-expression of CD133(+) and CD34(+) before and after isolation of MNCs were (0.18 +/- 0.11)% and (0.42 +/- 0.23)% respectively (p < 0.01); meanwhile about 90% of cells with CD133(+) expressed CD34 and 40% of cells with CD34(+) expressed CD133. It is concluded that the established method of detection using flow cytometry with three color fluorescence labelling can be used to detect expression of CD271, CD133 and CD34 in BMCs. The cells with CD271 are different from cells with CD133 and CD34, which suggests that the CD271 may be of important role in evaluating and guiding the clinical application of BM MSCs. FAU - Zhou, Jun AU - Zhou J AD - Department of Blood Transfusion, General Hospital, Beijing Military Area, Beijing 100700, China. zj990222@tom.com FAU - Zhu, Bing AU - Zhu B FAU - DU, Hai-Yan AU - DU HY FAU - Sun, Tian-Sheng AU - Sun TS FAU - Zhang, Chang-Hong AU - Zhang CH FAU - Yang, Lian-Gui AU - Yang LG LA - chi PT - Journal Article PL - China TA - Zhongguo Shi Yan Xue Ye Xue Za Zhi JT - Zhongguo shi yan xue ye xue za zhi JID - 101084424 RN - 0 (AC133 Antigen) RN - 0 (Antigens, CD) RN - 0 (Antigens, CD34) RN - 0 (Glycoproteins) RN - 0 (NGFR protein, human) RN - 0 (Nerve Tissue Proteins) RN - 0 (PROM1 protein, human) RN - 0 (Peptides) RN - 0 (Receptors, Nerve Growth Factor) SB - IM MH - AC133 Antigen MH - Antigens, CD/*metabolism MH - Antigens, CD34/*metabolism MH - Bone Marrow Cells/cytology/*metabolism MH - Cell Line MH - Flow Cytometry MH - Glycoproteins/*metabolism MH - Humans MH - Nerve Tissue Proteins/*metabolism MH - Peptides/*metabolism MH - Receptors, Nerve Growth Factor/*metabolism EDAT- 2009/02/25 09:00 MHDA- 2011/08/24 06:00 CRDT- 2009/02/25 09:00 PHST- 2009/02/25 09:00 [entrez] PHST- 2009/02/25 09:00 [pubmed] PHST- 2011/08/24 06:00 [medline] AID - 1009-2137(2009)01-0133-04 [pii] PST - ppublish SO - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009 Feb;17(1):133-6.