PMID- 19236848
OWN - NLM
STAT- MEDLINE
DCOM- 20090416
LR  - 20211203
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 381
IP  - 3
DP  - 2009 Apr 10
TI  - Hypoxia-induced expression of RTEF-1 (related transcriptional enhancer factor-1) 
      in endothelial cells is independent of HIF-1 (hypoxia-inducible factor-1).
PG  - 333-8
LID - 10.1016/j.bbrc.2009.02.083 [doi]
AB  - Related transcriptional enhancer factor-1 (RTEF-1) plays an important role in 
      transcriptional regulation of angiogenic genes in hypoxic endothelial cells. The 
      mechanisms involved in the induction of RTEF-1 expression in hypoxia are poorly 
      understood. In bovine aortic endothelial cells (BAEC) subjected to hypoxia, 
      Western blot and quantitative PCR analysis revealed that RTEF-1 protein and mRNA 
      levels were significantly increased by hypoxia. To address the potential role of 
      the hypoxia-inducible factor-1 (HIF-1) in RTEF-1 induction, a hepatoma cell line 
      deficient in HIF-1 (c4) and a control HIF-1 positive cell line (vT2) were 
      exposed to hypoxia. We report that RTEF-1 protein expression assessed by either 
      Western blotting or immunofluorescence was increased in both cell lines. This 
      demonstrates that HIF-1 is not required for RTEF-1 upregulation by hypoxia. 
      Conversely, RTEF-1 appeared to regulate the expression of HIF-1: HIF-1alpha 
      promoter activity was increased (3.6-fold) by RTEF-1 overexpression in BAEC. 
      Furthermore, RTEF-1 enhanced BAEC proliferation and tubule formation; these were 
      inhibited by RTEF-1 knockdown with siRNA. We propose that RTEF-1, acting via 
      HIF-1, is a key regulator of angiogenesis in response to hypoxia.
FAU - Zhang, Cuili
AU  - Zhang C
AD  - Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.
FAU - Song, Q H
AU  - Song QH
FAU - Li, Jian
AU  - Li J
FAU - Tian, Ye
AU  - Tian Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20090221
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Muscle Proteins)
RN  - 0 (TEA Domain Transcription Factors)
RN  - 0 (TEAD4 protein, human)
RN  - 0 (Transcription Factors)
SB  - IM
RIN - Biochem Biophys Res Commun. 2009 Oct 16;388(2):463. PMID: 19725178
MH  - Animals
MH  - Cattle
MH  - Cell Hypoxia
MH  - DNA-Binding Proteins/*biosynthesis
MH  - Endothelium, Vascular/*metabolism
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/*metabolism
MH  - Mice
MH  - Muscle Proteins/*biosynthesis
MH  - *Neovascularization, Physiologic/genetics
MH  - Promoter Regions, Genetic
MH  - TEA Domain Transcription Factors
MH  - Transcription Factors/*biosynthesis
EDAT- 2009/02/25 09:00
MHDA- 2009/04/17 09:00
CRDT- 2009/02/25 09:00
PHST- 2009/02/05 00:00 [received]
PHST- 2009/02/06 00:00 [accepted]
PHST- 2009/02/25 09:00 [entrez]
PHST- 2009/02/25 09:00 [pubmed]
PHST- 2009/04/17 09:00 [medline]
AID - S0006-291X(09)00280-0 [pii]
AID - 10.1016/j.bbrc.2009.02.083 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Apr 10;381(3):333-8. doi: 
      10.1016/j.bbrc.2009.02.083. Epub 2009 Feb 21.