PMID- 19237021
OWN - NLM
STAT- MEDLINE
DCOM- 20090407
LR  - 20231213
IS  - 1873-5487 (Electronic)
IS  - 0188-4409 (Linking)
VI  - 40
IP  - 2
DP  - 2009 Feb
TI  - Differential gene expression profiling of laryngeal squamous cell carcinoma by 
      laser capture microdissection and complementary DNA microarrays.
PG  - 114-23
LID - 10.1016/j.arcmed.2008.12.005 [doi]
AB  - BACKGROUND AND AIMS: Genetic alteration associated with initiation and 
      progression of laryngeal squamous cell carcinoma (LSCC) is largely unknown. The 
      aim of this study was to identify genetic changes associated with the disease 
      pathogenesis and pinpoint genes whose expression is impacted by these genetic 
      alterations. METHODS: Tumor cells were collected from eight matched pairs of 
      specimens of glottic carcinoma of the larynx and histologically normal epithelium 
      tissues adjacent to the carcinoma by laser capture microdissection (LCM). RNAs 
      prepared from these cells were used for genome-wide transcriptome analysis by 
      probing 16 cDNA microarrays. Real-time quantitative RT-PCR and 
      immunohistochemistry of tissue microarrays were used to validate a group of the 
      differentially expressed genes identified by the cDNA microarrays. RESULTS: 
      Hierarchical cluster analysis of the expressed genes showed that 2351 genes were 
      differentially expressed and could distinguish cancerous and noncancerous 
      samples. We also found 761 differentially expressed genes that were consistently 
      different between early stage and later stage specimens. Furthermore, abnormal 
      expression of some relevant genes such as MMP12, HMGA2, and TIMP4 were validated 
      by real-time quantitative RT-PCR and immunohistochemistry. Analysis of gene 
      ontology and pathway distributions then highlighted genes that may be critically 
      important to laryngeal carcinogenesis. CONCLUSIONS: Our results suggest that 
      using LCM plus DNA microarray analysis may facilitate the identification of 
      clinical molecular markers for disease and novel potential therapeutic targets 
      for LSCC.
FAU - Ma, Li-Juan
AU  - Ma LJ
AD  - Department of Otolaryngology Head/Neck Surgery, Hunan Provincial People's 
      Hospital, Changsha, PR China.
FAU - Li, Wei
AU  - Li W
FAU - Zhang, Xin
AU  - Zhang X
FAU - Huang, Dong-Hai
AU  - Huang DH
FAU - Zhang, Hua
AU  - Zhang H
FAU - Xiao, Jian-Yun
AU  - Xiao JY
FAU - Tian, Yong-Quan
AU  - Tian YQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Med Res
JT  - Archives of medical research
JID - 9312706
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HMGA2 Protein)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.65 (MMP12 protein, human)
RN  - EC 3.4.24.65 (Matrix Metalloproteinase 12)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Down-Regulation/genetics
MH  - Female
MH  - *Gene Expression Profiling
MH  - HMGA2 Protein/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*genetics
MH  - Male
MH  - Matrix Metalloproteinase 12/metabolism
MH  - Microdissection
MH  - Middle Aged
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Tissue Inhibitor of Metalloproteinases/metabolism
MH  - Up-Regulation/genetics
MH  - Tissue Inhibitor of Metalloproteinase-4
EDAT- 2009/02/25 09:00
MHDA- 2009/04/08 09:00
CRDT- 2009/02/25 09:00
PHST- 2008/07/13 00:00 [received]
PHST- 2008/12/01 00:00 [accepted]
PHST- 2009/02/25 09:00 [entrez]
PHST- 2009/02/25 09:00 [pubmed]
PHST- 2009/04/08 09:00 [medline]
AID - S0188-4409(08)00302-0 [pii]
AID - 10.1016/j.arcmed.2008.12.005 [doi]
PST - ppublish
SO  - Arch Med Res. 2009 Feb;40(2):114-23. doi: 10.1016/j.arcmed.2008.12.005.