PMID- 19238528
OWN - NLM
STAT- MEDLINE
DCOM- 20090617
LR  - 20220409
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 32
IP  - 2
DP  - 2009 Apr
TI  - Tetracyclines and chemically modified tetracycline-3 (CMT-3) modulate cytokine 
      secretion by lipopolysaccharide-stimulated whole blood.
PG  - 130-7
LID - 10.1007/s10753-009-9111-9 [doi]
AB  - In addition to their bacteriostatic effect, tetracyclines, which are often used 
      in the treatment of periodontitis, also present anti-inflammatory properties. In 
      the present study, we investigated the effects of tetracycline (TC), doxycycline 
      (doxy), and chemically modified tetracycline-3 (CMT-3) on the production of 
      pro-inflammatory mediators and matrix metalloproteinases (MMPs) in an ex vivo 
      human whole blood (WB) model stimulated with Porphyromonas gingivalis 
      lipopolysaccharide (LPS). WB samples obtained from three periodontitis patients 
      and six healthy subjects were stimulated with P. gingivalis LPS in the absence 
      and presence of TC, doxy, or CMT-3. The secretion of interleukin-1beta 
      (IL-1beta), interleukin-6 (IL-6), interleukin-8 (IL-8), MMP-8, and MMP-9 by the 
      WB samples was determined using enzyme-linked immunosorbent assays. P. gingivalis 
      LPS significantly increased the secretion of all cytokines and MMPs tested. While 
      we observed inter-patient variations, TC, doxy, and CMT-3 caused reductions of 
      LPS-induced cytokine secretion to various degrees. TC, doxy, and CMT-3 had no 
      significant effect on MMP-8 and MMP-9 secretion by LPS-stimulated WB samples. In 
      conclusion, we used a human WB model that takes into consideration relevant in 
      vivo immune cell interactions in the presence of plasma proteins to show that TC, 
      doxy, and CMT-3 can reduce the production of pro-inflammatory mediators. This 
      property may contribute to the clinically proven benefits of these molecules in 
      the treatment of periodontitis and other chronic inflammatory diseases.
FAU - Cazalis, Julia
AU  - Cazalis J
AD  - Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Universite 
      Laval, Quebec City, Quebec, G1K 7P4, Canada.
FAU - Tanabe, Shin-ichi
AU  - Tanabe S
FAU - Gagnon, Guy
AU  - Gagnon G
FAU - Sorsa, Timo
AU  - Sorsa T
FAU - Grenier, Daniel
AU  - Grenier D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tetracyclines)
RN  - 0 (tetracycline CMT-3)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - F8VB5M810T (Tetracycline)
RN  - N12000U13O (Doxycycline)
SB  - IM
MH  - Blood/*drug effects/immunology
MH  - Cytokines/drug effects/*metabolism
MH  - Doxycycline/pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Inflammation Mediators
MH  - Lipopolysaccharides/*pharmacology
MH  - Matrix Metalloproteinases/biosynthesis
MH  - Porphyromonas gingivalis
MH  - Tetracycline/*pharmacology
MH  - Tetracyclines/*pharmacology
MH  - Up-Regulation/drug effects
EDAT- 2009/02/25 09:00
MHDA- 2009/06/18 09:00
CRDT- 2009/02/25 09:00
PHST- 2009/02/25 09:00 [entrez]
PHST- 2009/02/25 09:00 [pubmed]
PHST- 2009/06/18 09:00 [medline]
AID - 10.1007/s10753-009-9111-9 [doi]
PST - ppublish
SO  - Inflammation. 2009 Apr;32(2):130-7. doi: 10.1007/s10753-009-9111-9.