PMID- 19239337
OWN - NLM
STAT- MEDLINE
DCOM- 20090529
LR  - 20090512
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 199
IP  - 6
DP  - 2009 Mar 15
TI  - Human cytomegalovirus-specific T cell reconstitution in young patients receiving 
      T cell-depleted, allogeneic hematopoietic stem cell transplantation.
PG  - 829-36
LID - 10.1086/597123 [doi]
AB  - BACKGROUND: Reconstitution of human cytomegalovirus (HCMV) T cell-specific 
      immunity is of crucial relevance in patients receiving a hematopoietic stem cell 
      transplant (HSCT). Scarce data on this subject are available for children 
      receiving a T cell-depleted HSCT. METHODS: We investigated HCMV-specific T cell 
      recovery in 48 recipients of a T cell-depleted HSCT from a human leukocyte 
      antigen (HLA)-disparate relative. Autologous HCMV-infected dendritic cells were 
      used to stimulate HCMV-specific CD8+ and CD4+ T cells producing interferon-gamma 
      (IFN). RESULTS: The 1-year cumulative incidence of both HCMV infection and 
      specific T cell reconstitution was 83% among the 23 HCMV-seropositive patients 
      and 4% and 8%, respectively, among the 25 HCMV-seronegative patients (P < .01). 
      HCMV-specific T cell reconstitution was significantly delayed in patients 
      receiving T cell-depleted grafts, compared with patients receiving unmanipulated 
      HSCTs (median time to reconstitution, 75 vs. 47 days, respectively; P < .01). The 
      median time from HCMV infection to immune recovery in recipients of T 
      cell-depleted grafts was 47 days. Detection of HCMV-specific T cells correlated 
      with control of HCMV infection. The number of residual T cells in the graft 
      predicted earlier T cell recovery (P = .02). CONCLUSIONS: Latent HCMV in the 
      recipient was the major cause of HCMV reactivation and also promoted specific T 
      cell reconstitution in patients given a T cell-depleted HSCT from an 
      HLA-disparate relative. Routine immunologic monitoring is valuable in identifying 
      patients with early HCMV-specific T cell reconstitution.
FAU - Lilleri, Daniele
AU  - Lilleri D
AD  - Servizio di Virologia, Fondazione Istituto di Ricovero e Cura a Carattere 
      Scientifico Policlinico San Matteo, Universita di Pavia, Pavia, Italy.
FAU - Gerna, Giuseppe
AU  - Gerna G
FAU - Fornara, Chiara
AU  - Fornara C
FAU - Chiesa, Antonella
AU  - Chiesa A
FAU - Comolli, Giuditta
AU  - Comolli G
FAU - Zecca, Marco
AU  - Zecca M
FAU - Locatelli, Franco
AU  - Locatelli F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Cytomegalovirus/*immunology
MH  - Cytomegalovirus Infections/*surgery
MH  - Female
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Lymphocyte Depletion
MH  - Male
MH  - Recurrence
MH  - T-Lymphocytes/*immunology
MH  - Transplantation, Homologous
MH  - Young Adult
EDAT- 2009/02/26 09:00
MHDA- 2009/05/30 09:00
CRDT- 2009/02/26 09:00
PHST- 2009/02/26 09:00 [entrez]
PHST- 2009/02/26 09:00 [pubmed]
PHST- 2009/05/30 09:00 [medline]
AID - 10.1086/597123 [doi]
PST - ppublish
SO  - J Infect Dis. 2009 Mar 15;199(6):829-36. doi: 10.1086/597123.