PMID- 19239424
OWN - NLM
STAT- MEDLINE
DCOM- 20090406
LR  - 20231213
IS  - 1600-0897 (Electronic)
IS  - 1046-7408 (Linking)
VI  - 61
IP  - 3
DP  - 2009 Mar
TI  - Effect of progesterone on HLA-E gene expression in JEG-3 choriocarcinoma cell 
      line.
PG  - 221-6
LID - 10.1111/j.1600-0897.2008.00684.x [doi]
AB  - PROBLEM: Among class Ib human leukocyte antigen (HLA) molecules, HLA-E is known 
      to be a major ligand of CD94/NKG2 receptor on natural killer (NK) cells, and to 
      play a pivotal role in recognition of extravillous trophoblasts (EVTs) by 
      maternal immune cells. However, it is scarcely known how HLA-E expression is 
      regulated in EVTs. METHOD OF STUDY: In this study, we investigated whether 
      progesterone, an essential hormone in maintaining pregnancy, regulated HLA-E 
      expression in EVT-like cell line, JEG-3. HLA-E mRNA amount in cultured JEG-3 
      cells was assessed by real-time PCR and cell-surface HLA-E protein was analyzed 
      by flowcytometry. RESULTS: Real-time PCR showed 3.5-fold increase 1 hour after 
      the addition of 1000 ng/ml progesterone. This response was diminished by the 
      addition of RU486, an antagonist for progesterone receptor. Flowcytometry 
      indicated that 1000 ng/ml progesterone slightly enhanced HLA-E expression on the 
      surface of JEG-3. CONCLUSION: These results suggest that progesterone 
      up-regulates HLA-E expression in JEG-3 cells through the pathway mediated by 
      progesterone receptor. Our findings might give a new insight into 
      immunomodulatory function of progesterone at fetomaternal interface.
FAU - Huang, Zhongying
AU  - Huang Z
AD  - Department of Obstetrics and Gynecology, The University of Tokyo, 7-3-1 Hongo, 
      Bunkyo, Tokyo, Japan.
FAU - Hyodo, Hironobu
AU  - Hyodo H
FAU - Fujii, Tomoyuki
AU  - Fujii T
FAU - Nagamatsu, Takeshi
AU  - Nagamatsu T
FAU - Matsumoto, Junko
AU  - Matsumoto J
FAU - Kawana, Kei
AU  - Kawana K
FAU - Yamashita, Takahiro
AU  - Yamashita T
FAU - Yasugi, Toshiharu
AU  - Yasugi T
FAU - Kozuma, Shiro
AU  - Kozuma S
FAU - Taketani, Yuji
AU  - Taketani Y
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Progesterone)
RN  - 320T6RNW1F (Mifepristone)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Cell Line, Tumor
MH  - Choriocarcinoma
MH  - Gene Expression/drug effects
MH  - *Gene Expression Regulation
MH  - HLA Antigens/*genetics
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Mifepristone/pharmacology
MH  - Progesterone/pharmacology/*physiology
MH  - RNA, Messenger/metabolism
MH  - Receptors, Progesterone/drug effects/*physiology
MH  - Trophoblasts/drug effects/*metabolism
MH  - Up-Regulation/drug effects
MH  - HLA-E Antigens
EDAT- 2009/02/26 09:00
MHDA- 2009/04/07 09:00
CRDT- 2009/02/26 09:00
PHST- 2009/02/26 09:00 [entrez]
PHST- 2009/02/26 09:00 [pubmed]
PHST- 2009/04/07 09:00 [medline]
AID - AJI684 [pii]
AID - 10.1111/j.1600-0897.2008.00684.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2009 Mar;61(3):221-6. doi: 10.1111/j.1600-0897.2008.00684.x.