PMID- 19239492
OWN - NLM
STAT- MEDLINE
DCOM- 20090708
LR  - 20220410
IS  - 1574-695X (Electronic)
IS  - 0928-8244 (Linking)
VI  - 56
IP  - 1
DP  - 2009 Jun
TI  - Purification and immunological characterization of a 12-kDa allergen from 
      Epicoccum purpurascens.
PG  - 32-40
LID - 10.1111/j.1574-695X.2009.00542.x [doi]
AB  - Exposure to Epicoccum purpurascens is implicated in respiratory allergies and 
      asthma. Several allergens of clinical importance were identified in Epicoccum 
      extract (EE), but only one allergen has been isolated and characterized. In the 
      present study, a 12-kDa allergen was isolated from an Epicoccum spore-mycelial 
      extract by concanavalin-A sepharose, reverse-phase hydrophobic and gel filtration 
      chromatography. The purified protein was recognized as a single 12-kDa allergen 
      on immunoblot with a serum pool of Epicoccum-sensitive patients. Of the 94 
      respiratory allergy patients tested intradermally, 17 showed marked positive skin 
      reactions to EE and 12 of them reacted with the 12-kDa protein, indicating a 
      diagnostic sensitivity of 70%. More than 80% patients' sera showed immunoglobulin 
      E (IgE) reactivity to the purified protein in enzyme-linked immunosorbent assay 
      and immunoblot, identifying it as a major allergen. Preincubation of pooled serum 
      with the protein led to inhibition of IgE binding to solid-phase-bound EE 
      (effective concentration 50%=180 ng). Twelve of the 17 serum samples showed 
      significant basophil histamine release upon stimulation with purified protein. 
      The protein induced significant proliferation of peripheral blood mononuclear 
      cells of 13 patients. A high level of interleukin-4 in the culture supernatant of 
      these cells indicated induction of a T-helper type 2 response. The purified 
      12-kDa protein is a clinically relevant allergen and has potential for the 
      diagnosis and therapy of Epicoccum allergies.
FAU - Kukreja, Neetu
AU  - Kukreja N
AD  - Allergy and Immunology Section, Institute of Genomics and Integrative Biology 
      (CSIR), Delhi University Campus, Delhi, India.
FAU - Sridhara, Susheela
AU  - Sridhara S
FAU - Singh, Bhanu Pratap
AU  - Singh BP
FAU - Gaur, Shailendra Nath
AU  - Gaur SN
FAU - Arora, Naveen
AU  - Arora N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090223
PL  - England
TA  - FEMS Immunol Med Microbiol
JT  - FEMS immunology and medical microbiology
JID - 9315554
RN  - 0 (Allergens)
RN  - 0 (Fungal Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/chemistry/*immunology/*isolation & purification
MH  - Ascomycota/*immunology
MH  - Fungal Proteins/chemistry/*immunology/*isolation & purification
MH  - Hemagglutination Inhibition Tests
MH  - Histamine Release
MH  - Humans
MH  - Hypersensitivity/blood/immunology/*microbiology
MH  - Immunoglobulin E/blood
MH  - Intradermal Tests
MH  - Lymphocyte Activation
MH  - Molecular Weight
MH  - Tandem Mass Spectrometry
EDAT- 2009/02/26 09:00
MHDA- 2009/07/09 09:00
CRDT- 2009/02/26 09:00
PHST- 2009/02/26 09:00 [entrez]
PHST- 2009/02/26 09:00 [pubmed]
PHST- 2009/07/09 09:00 [medline]
AID - FIM542 [pii]
AID - 10.1111/j.1574-695X.2009.00542.x [doi]
PST - ppublish
SO  - FEMS Immunol Med Microbiol. 2009 Jun;56(1):32-40. doi: 
      10.1111/j.1574-695X.2009.00542.x. Epub 2009 Feb 23.