PMID- 19240255
OWN - NLM
STAT- MEDLINE
DCOM- 20090716
LR  - 20220316
IS  - 0193-1849 (Print)
IS  - 1522-1555 (Electronic)
IS  - 0193-1849 (Linking)
VI  - 296
IP  - 6
DP  - 2009 Jun
TI  - RANTES release by human adipose tissue in vivo and evidence for depot-specific 
      differences.
PG  - E1262-8
LID - 10.1152/ajpendo.90511.2008 [doi]
AB  - Obesity is associated with elevated inflammatory signals from various adipose 
      tissue depots. This study aimed to evaluate release of regulated on activation, 
      normal T cell expressed and secreted (RANTES) by human adipose tissue in vivo and 
      ex vivo, in reference to monocyte chemoattractant protein-1 (MCP-1) and 
      interleukin-6 (IL-6) release. Arteriovenous differences of RANTES, MCP-1, and 
      IL-6 were studied in vivo across the abdominal subcutaneous adipose tissue in 
      healthy Caucasian subjects with a wide range of adiposity. Systemic levels and ex 
      vivo RANTES release were studied in abdominal subcutaneous, gastric fat pad, and 
      omental adipose tissue from morbidly obese bariatric surgery patients and in 
      thoracic subcutaneous and epicardial adipose tissue from cardiac surgery patients 
      without coronary artery disease. Arteriovenous studies confirmed in vivo RANTES 
      and IL-6 release in adipose tissue of lean and obese subjects and release of 
      MCP-1 in obesity. However, in vivo release of MCP-1 and RANTES, but not IL-6, was 
      lower than circulating levels. Ex vivo release of RANTES was greater from the 
      gastric fat pad compared with omental (P = 0.01) and subcutaneous (P = 0.001) 
      tissue. Epicardial adipose tissue released less RANTES than thoracic subcutaneous 
      adipose tissue in lean (P = 0.04) but not obese subjects. Indexes of obesity 
      correlated with epicardial RANTES but not with systemic RANTES or its release 
      from other depots. In conclusion, RANTES is released by human subcutaneous 
      adipose tissue in vivo and in varying amounts by other depots ex vivo. While it 
      appears unlikely that the adipose organ contributes significantly to circulating 
      levels, local implications of this chemokine deserve further investigation.
FAU - Madani, Rana
AU  - Madani R
AD  - Centre for Clinical Pharmacology, Div. of Medicine, University College London, 5 
      University St., London, UK WC1 6JJ.
FAU - Karastergiou, Kalypso
AU  - Karastergiou K
FAU - Ogston, Nicola C
AU  - Ogston NC
FAU - Miheisi, Nazar
AU  - Miheisi N
FAU - Bhome, Rahul
AU  - Bhome R
FAU - Haloob, Nora
AU  - Haloob N
FAU - Tan, Garry D
AU  - Tan GD
FAU - Karpe, Fredrik
AU  - Karpe F
FAU - Malone-Lee, James
AU  - Malone-Lee J
FAU - Hashemi, Majid
AU  - Hashemi M
FAU - Jahangiri, Marjan
AU  - Jahangiri M
FAU - Mohamed-Ali, Vidya
AU  - Mohamed-Ali V
LA  - eng
GR  - 078055/Z/05/Z/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090224
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCL5 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Arteries/metabolism
MH  - Body Weight
MH  - Chemokine CCL2/blood
MH  - Chemokine CCL5/*blood/genetics
MH  - Female
MH  - Humans
MH  - Interleukin-6/blood
MH  - Intra-Abdominal Fat/blood supply/immunology/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Obesity, Morbid/*immunology/*metabolism
MH  - Omentum/blood supply/immunology/metabolism
MH  - Organ Culture Techniques
MH  - RNA, Messenger/metabolism
MH  - Subcutaneous Fat, Abdominal/blood supply/immunology/*metabolism
MH  - Veins/metabolism
PMC - PMC2692396
EDAT- 2009/02/26 09:00
MHDA- 2009/07/17 09:00
PMCR- 2010/06/01
CRDT- 2009/02/26 09:00
PHST- 2009/02/26 09:00 [entrez]
PHST- 2009/02/26 09:00 [pubmed]
PHST- 2009/07/17 09:00 [medline]
PHST- 2010/06/01 00:00 [pmc-release]
AID - 90511.2008 [pii]
AID - E-90511-2008 [pii]
AID - 10.1152/ajpendo.90511.2008 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2009 Jun;296(6):E1262-8. doi: 
      10.1152/ajpendo.90511.2008. Epub 2009 Feb 24.