PMID- 1924170
OWN - NLM
STAT- MEDLINE
DCOM- 19911105
LR  - 20190818
IS  - 0724-8741 (Print)
IS  - 0724-8741 (Linking)
VI  - 8
IP  - 8
DP  - 1991 Aug
TI  - Column-switching high-performance liquid chromatography for on-line simultaneous 
      determination and resolution of enantiomers of verapamil and its metabolites.
PG  - 997-1001
AB  - An on-line simultaneous assay for the enantiomers of verapamil (VA) and its three 
      metabolites in plasma was developed with column-switching HPLC. This system 
      consists of an ovomucoid protein chiral stationary phase coupled to an achiral 
      reversed-phase column via a dilution tube and a trapping column. The 
      reversed-phase column was used to separate and quantitate VA, its metabolites, 
      and internal standard, without interference from plasma components. Then each of 
      the eluates containing VA and its metabolites was selectively switched into a 
      sampling loop, and the samples were transferred successively to the trapping 
      column after dilution with a new mobile phase. After concentration on the 
      trapping column, each sample was passed to the ovomucoid column, where the 
      enantiomers were resolved and quantitated. The mobile phases for both HPLC 
      columns were independently optimized and the diffusion of the sample during 
      column switching was minimized. This method was shown to be efficient and 
      reliable.
FAU - Oda, Y
AU  - Oda Y
AD  - Department of Physical and Analytical Chemistry, Tsukuba Research Laboratories, 
      Eisai Co., Ltd., Ibaraki, Japan.
FAU - Asakawa, N
AU  - Asakawa N
FAU - Kajima, T
AU  - Kajima T
FAU - Yoshida, Y
AU  - Yoshida Y
FAU - Sato, T
AU  - Sato T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - CJ0O37KU29 (Verapamil)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Stereoisomerism
MH  - Verapamil/*blood
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
AID - 10.1023/a:1015848806240 [doi]
PST - ppublish
SO  - Pharm Res. 1991 Aug;8(8):997-1001. doi: 10.1023/a:1015848806240.