PMID- 19243502
OWN - NLM
STAT- MEDLINE
DCOM- 20090505
LR  - 20131121
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 48
IP  - 4
DP  - 2009 Apr
TI  - Molecular ecology of a facultative swine waste lagoon.
PG  - 486-92
LID - 10.1111/j.1472-765X.2009.02560.x [doi]
AB  - AIMS: To investigate the microbial ecology of three facultative swine waste 
      lagoons. METHODS AND RESULTS: Phylogenetic analysis of sequences in a 16S rRNA 
      gene clone library and fluorescence in situ hybridization (FISH) analyses were 
      used to assess bacterial diversity in a swine waste lagoon. FISH analysis and 
      Gram-staining were used to compare the microbial communities of all three swine 
      waste lagoons. Six operational taxonomic units were in high relative abundance 
      and corresponded to the following phylotypes; Thiolamprovum, Verrucomicrobia, 
      Acholeplasma, Turicibacter, Clostridium and Bacteroides. PCR was employed to 
      detect the genes apsA and dsrAB which encode for enzymes specifically associated 
      with dissimilatory sulfate-reduction within sulfate-reducing bacteria (SRB). 
      Amplification of these genes confirmed their presence within the lagoons. 
      CONCLUSIONS: All lagoons were dominated by purple sulfur bacteria, affiliated to 
      Thiolamprovum pedioforme. The molecular identification of fermentative bacteria 
      and SRB indicate the following metabolic processes within such facultative ponds: 
      sulfur-cycling, fermentation, inter-species hydrogen transfer and carbon cycling. 
      SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides the first molecular 
      evidence for the existence of a sulfur cycle which is linked to phototrophic 
      sulfide oxidation by purple bacteria and organotrophic sulfate-reduction by SRB.
FAU - Goh, S H M
AU  - Goh SH
AD  - School of Molecular and Microbial Sciences, University of Queensland, St Lucia, 
      Brisbane, QLD, Australia.
FAU - Mabbett, A N
AU  - Mabbett AN
FAU - Welch, J P
AU  - Welch JP
FAU - Hall, S J
AU  - Hall SJ
FAU - McEwan, A G
AU  - McEwan AG
LA  - eng
SI  - GENBANK/DQ901401
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090223
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sulfates)
RN  - 70FD1KFU70 (Sulfur)
RN  - 7440-44-0 (Carbon)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Animal Husbandry/methods
MH  - Animals
MH  - *Bacteria/classification/genetics/isolation & purification
MH  - Carbon/metabolism
MH  - Chromatiaceae/classification/genetics/isolation & purification
MH  - *Ecosystem
MH  - Gene Library
MH  - Genes, rRNA
MH  - Hydrogen/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sulfates/metabolism
MH  - Sulfur/*metabolism
MH  - *Swine
MH  - Waste Disposal, Fluid/*methods
MH  - *Water Microbiology
EDAT- 2009/02/27 09:00
MHDA- 2009/05/06 09:00
CRDT- 2009/02/27 09:00
PHST- 2009/02/27 09:00 [entrez]
PHST- 2009/02/27 09:00 [pubmed]
PHST- 2009/05/06 09:00 [medline]
AID - LAM2560 [pii]
AID - 10.1111/j.1472-765X.2009.02560.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2009 Apr;48(4):486-92. doi: 
      10.1111/j.1472-765X.2009.02560.x. Epub 2009 Feb 23.