PMID- 19244318
OWN - NLM
STAT- MEDLINE
DCOM- 20090428
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 83
IP  - 10
DP  - 2009 May
TI  - The 30-amino-acid deletion in the Nsp2 of highly pathogenic porcine reproductive 
      and respiratory syndrome virus emerging in China is not related to its virulence.
PG  - 5156-67
LID - 10.1128/JVI.02678-08 [doi]
AB  - During the past 2 years, an atypical clinical outbreak, caused by a highly 
      pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) with a 
      unique 30-amino-acid deletion in its Nsp2-coding region, was pandemic in China. 
      In this study, we generated four full-length infectious cDNA clones: a clone of 
      the highly virulent PRRSV strain JXwn06 (pWSK-JXwn), a clone of the low-virulence 
      PRRSV strain HB-1/3.9 (pWSK-HB-1/3.9), a chimeric clone in which the Nsp2 region 
      containing the 30-amino-acid deletion was replaced by the corresponding region of 
      the low-virulence PRRSV strain HB-1/3.9 (pWSK-JXwn-HB1nsp2), and a mutated 
      HB-1/3.9 clone with the same deletion in Nsp2 as JXwn06 (pWSK-HB1-ND30). We also 
      investigated the pathogenicities of the rescued viruses (designated RvJXwn, 
      RvJXwn-HB1nsp2, RvHB-1/3.9, and RvHB1-ND30, respectively) in 
      specific-pathogen-free piglets in order to determine the role of the 
      30-amino-acid deletion in the virulence of the highly pathogenic PRRSV. All the 
      rescued viruses could replicate stably in MARC-145 cells. Our findings indicated 
      that RvJXwn-HB1nsp2 retained high virulence for piglets, like RvJXwn and the 
      parental virus JXwn06, although the survival time of piglets infected with 
      RvJXwn-HB1nsp2 was obviously prolonged. RvHB1-ND30 exhibited low virulence for 
      piglets, like RvHB-1/3.9 and the parental virus HB-1/3.9. Therefore, we conclude 
      that the 30-amino-acid deletion is not related to the virulence of the highly 
      pathogenic PRRSV emerging in China.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary 
      Medicine, China Agricultural University, Beijing 100193, People's Republic of 
      China.
FAU - Zhang, Jialong
AU  - Zhang J
FAU - Zeng, Jingwen
AU  - Zeng J
FAU - Yin, Shuoyan
AU  - Yin S
FAU - Li, Yanhua
AU  - Li Y
FAU - Zheng, Linying
AU  - Zheng L
FAU - Guo, Xin
AU  - Guo X
FAU - Ge, Xinna
AU  - Ge X
FAU - Yang, Hanchun
AU  - Yang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090225
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - China
MH  - DNA, Complementary/genetics
MH  - Genome, Viral
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/pathogenicity/physiology
MH  - RNA, Viral/genetics
MH  - Sequence Alignment
MH  - *Sequence Deletion
MH  - Sus scrofa/virology
MH  - Viral Nonstructural Proteins/*genetics
MH  - Virulence
MH  - Virus Replication
PMC - PMC2682102
EDAT- 2009/02/27 09:00
MHDA- 2009/04/29 09:00
PMCR- 2009/11/01
CRDT- 2009/02/27 09:00
PHST- 2009/02/27 09:00 [entrez]
PHST- 2009/02/27 09:00 [pubmed]
PHST- 2009/04/29 09:00 [medline]
PHST- 2009/11/01 00:00 [pmc-release]
AID - JVI.02678-08 [pii]
AID - 2678-08 [pii]
AID - 10.1128/JVI.02678-08 [doi]
PST - ppublish
SO  - J Virol. 2009 May;83(10):5156-67. doi: 10.1128/JVI.02678-08. Epub 2009 Feb 25.