PMID- 19246895 OWN - NLM STAT- MEDLINE DCOM- 20090409 LR - 20090312 IS - 1421-9743 (Electronic) IS - 0006-8977 (Linking) VI - 73 IP - 1 DP - 2009 TI - Brain-derived neurotrophic factor mRNA expression in the brain of the teleost fish, Anguilla anguilla, the European Eel. PG - 43-58 LID - 10.1159/000204962 [doi] AB - The spatial expression pattern of Brain-Derived Neurotrophic Factor (BDNF) mRNA in the brain of the European eel (Anguilla anguilla) was determined using non-radioactive in situ hybridization, and mapped and compared to that of other vertebrates. Riboprobes were prepared based on a partial cDNA coding sequence for A. anguilla BDNF that was amplified using degenerate primers, cloned and sequenced. As in other animal groups, in the eel, BDNF mRNA expression was seen in the telencephalon, hypothalamus, tectum, many primary and secondary sensory centers, and cranial motor nuclei. However, in contrast to mammals, BDNF mRNA expression was observed in some brain stem nuclei, such as the reticular formation, that contain cell bodies of neurons that project down the spinal cord. We suggest that these differences might relate to the continual growth of teleost fish. CI - Copyright 2009 S. Karger AG, Basel. FAU - Dalton, Victoria S AU - Dalton VS AD - Department of Zoology, Trinity College, University of Dublin, Dublin, Ireland. victoria.dalton@ansto.gov.au FAU - Borich, Suzanne M AU - Borich SM FAU - Murphy, Paula AU - Murphy P FAU - Roberts, Barry L AU - Roberts BL LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090227 PL - Switzerland TA - Brain Behav Evol JT - Brain, behavior and evolution JID - 0151620 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Messenger) SB - IM MH - Amino Acid Sequence MH - Anguilla/*metabolism MH - Animals MH - Brain/*metabolism MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Conserved Sequence MH - In Situ Hybridization MH - Molecular Sequence Data MH - Photomicrography MH - RNA, Messenger/*metabolism MH - Rats MH - Spinal Cord/metabolism EDAT- 2009/02/28 09:00 MHDA- 2009/04/10 09:00 CRDT- 2009/02/28 09:00 PHST- 2008/01/29 00:00 [received] PHST- 2008/02/27 00:00 [accepted] PHST- 2009/02/28 09:00 [entrez] PHST- 2009/02/28 09:00 [pubmed] PHST- 2009/04/10 09:00 [medline] AID - 000204962 [pii] AID - 10.1159/000204962 [doi] PST - ppublish SO - Brain Behav Evol. 2009;73(1):43-58. doi: 10.1159/000204962. Epub 2009 Feb 27.