PMID- 19249068
OWN - NLM
STAT- MEDLINE
DCOM- 20090727
LR  - 20220321
IS  - 1878-3503 (Electronic)
IS  - 0035-9203 (Linking)
VI  - 103
IP  - 4
DP  - 2009 Apr
TI  - Evaluation of reverse-transcriptase PCR as a diagnostic tool to confirm Japanese 
      encephalitis virus infection.
PG  - 403-6
LID - 10.1016/j.trstmh.2009.01.021 [doi]
AB  - Japanese encephalitis (JE) is a serious central nervous system infection and 
      major public health problem in several countries of Southeast Asia including 
      India. This study evaluated the use of IgM ELISA and reverse-transcriptase 
      (RT)-PCR in blood and cerebrospinal fluid (CSF) samples from acute encephalitis 
      patients for the detection of Japanese encephalitis virus (JEV). Forty-four 
      children suffering from acute encephalitis were enrolled, and 36 were selected 
      from whom both CSF and serum samples were available. Twenty-two of the 36 CSF 
      samples were positive for JEV by IgM ELISA and all were negative by RT-PCR. 
      Twenty-three of the 36 serum samples were positive by IgM ELISA while 28 were 
      positive by RT-PCR. Total positivity for JEV infection in CSF and serum samples 
      was 66.7% (24/36) and 83.3% (30/36) respectively by one or both tests. The 
      overall positivity for JEV infection was 86.1% (31/36). We suggest that the use 
      of RT-PCR in serum samples during the early days of JEV infection may be helpful 
      in confirming diagnosis in those cases which are negative for JEV-specific IgM 
      antibodies in both serum and CSF samples.
FAU - Saxena, Vandana
AU  - Saxena V
AD  - Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical 
      Sciences, Lucknow 226 014, India.
FAU - Mishra, Virendra K
AU  - Mishra VK
FAU - Dhole, Tapan N
AU  - Dhole TN
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090226
PL  - England
TA  - Trans R Soc Trop Med Hyg
JT  - Transactions of the Royal Society of Tropical Medicine and Hygiene
JID - 7506129
RN  - 0 (Immunoglobulin M)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Encephalitis Virus, Japanese/*immunology
MH  - Encephalitis, Japanese/*diagnosis/immunology
MH  - Enzyme-Linked Immunosorbent Assay/methods/standards
MH  - Humans
MH  - Immunoglobulin M/blood/*cerebrospinal fluid
MH  - India
MH  - Infant
MH  - RNA, Viral/blood/cerebrospinal fluid
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods/standards
MH  - Sensitivity and Specificity
EDAT- 2009/03/03 09:00
MHDA- 2009/07/28 09:00
CRDT- 2009/03/03 09:00
PHST- 2006/10/08 00:00 [received]
PHST- 2009/01/22 00:00 [revised]
PHST- 2009/01/22 00:00 [accepted]
PHST- 2009/03/03 09:00 [entrez]
PHST- 2009/03/03 09:00 [pubmed]
PHST- 2009/07/28 09:00 [medline]
AID - S0035-9203(09)00022-4 [pii]
AID - 10.1016/j.trstmh.2009.01.021 [doi]
PST - ppublish
SO  - Trans R Soc Trop Med Hyg. 2009 Apr;103(4):403-6. doi: 
      10.1016/j.trstmh.2009.01.021. Epub 2009 Feb 26.