PMID- 19249125
OWN - NLM
STAT- MEDLINE
DCOM- 20091013
LR  - 20171116
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 214
IP  - 8
DP  - 2009
TI  - Effect of nitric oxide donors on NADPH oxidase signaling pathway in human 
      neutrophils in vitro.
PG  - 692-702
LID - 10.1016/j.imbio.2008.12.001 [doi]
AB  - The production of reactive oxygen species (ROS) in activated neutrophils is 
      catalyzed by NADPH oxidase, a multiproteins complex. Various protein kinases 
      including protein kinase C (PKC) and mitogen activated protein kinases (MAPKs) 
      are involved in NADPH oxidase phosphorylation and activation. The main step in 
      the NADPH oxidase activation is phosphorylation of its cytosolic protein 
      p47(phox). We found previously that nitric oxide (NO) donors such as metabolite 
      of molsidomine-SIN-1 and diethylamine/NO significantly impaired the ROS 
      production in activated human neutrophils. In this study, we investigated the 
      effects of both NO donors on NADPH oxidase-linked signaling proteins in activated 
      neutrophils. We found that NO donors decreased the phosphorylation of p47(phox) 
      on tyrosine and serine/threonine residues and PKC on serine residues in 
      neutrophils. Both NO donors did not affect the phosphorylation of MAPKs. NO 
      donors partially but significantly lost their ability to reduce ROS production in 
      the presence of PKC but not MAPKs inhibitors. We show that whereas NO donors have 
      no effect on MAPKs activity, they do decrease PMA- and/or fMLP-induced 
      phosphorylation of p47(phox) and PKC as well as PMA- and fMLP-induced ROS 
      production.
FAU - Klink, Magdalena
AU  - Klink M
AD  - Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232 
      Lodz, Poland. mklink@cbm.pan.pl
FAU - Jastrzembska, Katarzyna
AU  - Jastrzembska K
FAU - Bednarska, Katarzyna
AU  - Bednarska K
FAU - Banasik, Malgorzata
AU  - Banasik M
FAU - Sulowska, Zofia
AU  - Sulowska Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090226
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Diethylamines)
RN  - 0 (Nitric Oxide Donors)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 5O5U71P6VQ (linsidomine)
RN  - D46583G77X (Molsidomine)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.3.1 (neutrophil cytosolic factor 1)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Diethylamines/immunology/metabolism
MH  - Enzyme Activation
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Humans
MH  - Molsidomine/*analogs & derivatives/*antagonists & inhibitors/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/immunology/metabolism
MH  - NADPH Oxidases/immunology/*metabolism
MH  - Neutrophils
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Donors/*pharmacology
MH  - Phosphorylation/drug effects/immunology
MH  - Protein Kinase C/metabolism
MH  - *Signal Transduction/drug effects/immunology
MH  - Substrate Specificity
EDAT- 2009/03/03 09:00
MHDA- 2009/10/14 06:00
CRDT- 2009/03/03 09:00
PHST- 2008/07/14 00:00 [received]
PHST- 2008/12/05 00:00 [revised]
PHST- 2008/12/08 00:00 [accepted]
PHST- 2009/03/03 09:00 [entrez]
PHST- 2009/03/03 09:00 [pubmed]
PHST- 2009/10/14 06:00 [medline]
AID - S0171-2985(08)00163-0 [pii]
AID - 10.1016/j.imbio.2008.12.001 [doi]
PST - ppublish
SO  - Immunobiology. 2009;214(8):692-702. doi: 10.1016/j.imbio.2008.12.001. Epub 2009 
      Feb 26.