PMID- 19249917
OWN - NLM
STAT- MEDLINE
DCOM- 20100824
LR  - 20210115
IS  - 0862-8408 (Print)
IS  - 0862-8408 (Linking)
VI  - 59
IP  - 1
DP  - 2010
TI  - The influence of obesity and different fat depots on adipose tissue gene 
      expression and protein levels of cell adhesion molecules.
PG  - 79-88
AB  - Increased circulating adhesion molecules in patients with obesity play an 
      important role in the development of endothelial dysfunction/atherosclerosis. The 
      aim of this study was to assess the contribution of various fat depots to the 
      production of adhesion molecules in obesity. 12 women with first and second 
      degree of obesity, 13 women with third degree of obesity and 14 lean age-matched 
      women were included into study. Circulating levels of vascular cell adhesion 
      molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin 
      were measured by Luminex kits. mRNA expression of ICAM-1, VCAM-1, E-selectin, 
      monocyte chemoattractant protein-1 (MCP-1), and CD68 in subcutaneous (SAT) and 
      visceral adipose tissue (VAT) was measured by RT-PCR; ICAM-1 and VCAM-1 protein 
      levels by Luminex kits, normalized to protein content. Obesity increased ICAM-1 
      and VCAM-1 mRNA expression and protein levels and CD68 mRNA expression in VAT. 
      Expression of E-selectin and MCP-1 did not significantly differ between groups. 
      Expression of ICAM-1 and VCAM-1 positively correlated with expression of CD68 in 
      both adipose depots. In VAT, ICAM-1 and VCAM-1 expression and protein levels 
      positively correlated with BMI. Obesity was associated with increased adhesion 
      molecules mRNA expression and protein levels in VAT, but not in SAT. Increased 
      adhesion molecules production in visceral fat may provide a novel direct link 
      between visceral adiposity and increased risk of cardiovascular complications.
FAU - Bosanska, L
AU  - Bosanska L
AD  - Third Department of Medicine, First Faculty of Medicine, Charles University, 
      Prague, Czech Republic.
FAU - Michalsky, D
AU  - Michalsky D
FAU - Lacinova, Z
AU  - Lacinova Z
FAU - Dostalova, I
AU  - Dostalova I
FAU - Bartlova, M
AU  - Bartlova M
FAU - Haluzikova, D
AU  - Haluzikova D
FAU - Matoulek, M
AU  - Matoulek M
FAU - Kasalicky, M
AU  - Kasalicky M
FAU - Haluzik, M
AU  - Haluzik M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090227
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (E-Selectin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - *Adiposity
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - Biomarkers/metabolism
MH  - Body Mass Index
MH  - Cardiovascular Diseases/etiology/metabolism/physiopathology
MH  - Case-Control Studies
MH  - Cell Adhesion Molecules/blood/genetics/*metabolism
MH  - Chemokine CCL2/metabolism
MH  - E-Selectin/metabolism
MH  - Female
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Intra-Abdominal Fat/*metabolism/physiopathology
MH  - Middle Aged
MH  - Obesity/complications/genetics/*metabolism/physiopathology
MH  - RNA, Messenger/blood
MH  - Severity of Illness Index
MH  - Subcutaneous Fat, Abdominal/*metabolism/physiopathology
MH  - Up-Regulation
MH  - Vascular Cell Adhesion Molecule-1/metabolism
EDAT- 2009/03/03 09:00
MHDA- 2010/08/25 06:00
CRDT- 2009/03/03 09:00
PHST- 2009/03/03 09:00 [entrez]
PHST- 2009/03/03 09:00 [pubmed]
PHST- 2010/08/25 06:00 [medline]
AID - 1705 [pii]
AID - 10.33549/physiolres.931705 [doi]
PST - ppublish
SO  - Physiol Res. 2010;59(1):79-88. doi: 10.33549/physiolres.931705. Epub 2009 Feb 27.