PMID- 19250212
OWN - NLM
STAT- MEDLINE
DCOM- 20090330
LR  - 20141120
IS  - 1749-6632 (Electronic)
IS  - 0077-8923 (Linking)
VI  - 1155
DP  - 2009 Feb
TI  - Aromatase and amphiregulin are correspondingly expressed in human liver cancer 
      cells.
PG  - 252-6
LID - 10.1111/j.1749-6632.2009.03695.x [doi]
AB  - Human hepatocellular carcinoma (HCC) is associated with high mortality rates, 
      being the third most common cause of cancer death worldwide. Although estrogens 
      have been implicated in HCC, their potential role in development and/or 
      progression of this malignancy remains unclear. In this study we investigated 
      mRNA and protein expression of aromatase (Aro) and amphiregulin (AREG) in 
      relation to estrogen receptors (ERs), in HepG2, Huh7, and HA22T human malignant 
      liver cell lines, using RT-PCR and Western blot analyses. Aro expression was 
      significantly higher (approximately 13-fold, P= 0.003) in HepG2 cells than in 
      Huh7 cells, while no Aro expression could be detected in HA22T cells. 
      Interestingly, the patterns of AREG expression were consistently associated with 
      those of Aro, with approximately 3-fold and approximately 8-fold higher levels 
      being seen in HepG2 cells than in Huh7 cells (P= 0.002) and HA22T cells (P= 
      0.0014), respectively. Using a specific set of primers, ERalpha46 is the only ER 
      variant expressed in all cell lines, while the wild-type ERalpha66 could not be 
      detected. Western blot analysis revealed a corresponding figure. This evidence 
      suggests that AREG expression may be upregulated by estrogens in human HCC and 
      that locally elevated aromatase activity also may increase malignant cell 
      proliferation through AREG signaling.
FAU - Miceli, Vitale
AU  - Miceli V
AD  - Experimental Oncology Unit, Department of Oncology, ARNAS-Civico, Palermo, Italy.
FAU - Cervello, Melchiorre
AU  - Cervello M
FAU - Azzolina, Antonina
AU  - Azzolina A
FAU - Montalto, Giuseppe
AU  - Montalto G
FAU - Calabro, Maurizio
AU  - Calabro M
FAU - Carruba, Giuseppe
AU  - Carruba G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (DNA Primers)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - Amphiregulin
MH  - Aromatase/*metabolism
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - DNA Primers
MH  - EGF Family of Proteins
MH  - Estrogen Receptor alpha/genetics/metabolism
MH  - Glycoproteins/*metabolism
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*metabolism
MH  - Liver Neoplasms/enzymology/*metabolism/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2009/03/03 09:00
MHDA- 2009/03/31 09:00
CRDT- 2009/03/03 09:00
PHST- 2009/03/03 09:00 [entrez]
PHST- 2009/03/03 09:00 [pubmed]
PHST- 2009/03/31 09:00 [medline]
AID - NYAS03695 [pii]
AID - 10.1111/j.1749-6632.2009.03695.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2009 Feb;1155:252-6. doi: 10.1111/j.1749-6632.2009.03695.x.