PMID- 19252227
OWN - NLM
STAT- MEDLINE
DCOM- 20090713
LR  - 20181201
IS  - 0006-355X (Print)
IS  - 0006-355X (Linking)
VI  - 46
IP  - 1
DP  - 2009
TI  - Effect of TGF beta1, BMP-2 and hydraulic pressure on chondrogenic differentiation 
      of bovine bone marrow mesenchymal stromal cells.
PG  - 45-55
LID - 10.3233/BIR-2009-0520 [doi]
AB  - Bioactive factors, such as TGF beta and BMP-2, as well as mechanical factors i.e. 
      compressive loading and hydraulic pressure, have been shown to induce and/or 
      modulate chondrogenesis of bone marrow derived mesenchymal stromal cells (BMSCs). 
      Since these factors are intracellularly transduced through different mechanisms, 
      it is hypothesized that TGF beta, BMP-2 and hydraulic pressure may act 
      synergistically on chondrogenic differentiation of BMSCs. Aggregates of bovine 
      BMSC were cultured in the presence of 10 ng/ml TGF beta1, 50 ng/ml BMP-2 or both. 
      Half of the samples were loaded for 4 hours per day with 0.5-3 MPa cyclic 
      hydraulic pressure at 1 Hz. After 14 days of culture/loading, gene expression of 
      chondrogenic genes was assessed. DNA as well as glycosaminoglycan (GAG) content 
      of the pellets were analysed. Neither pressure nor BMP-2 had an influence on 
      GAG/DNA content. However, cells responded to the presence of TGF beta1 with an 
      up-regulation of chondrogenic genes and GAG/DNA of the aggregates increased 
      compared to controls demonstrating the cells ability to respond to external 
      stimuli. The used concentrations of BMP-2 and parameters for pressure were 
      neither able to induce nor modulate chondrogenesis of bovine BMSCs and thus no 
      synergistic effects were observed.
FAU - Zeiter, Stephan
AU  - Zeiter S
AD  - AO Research Institute, Davos, Switzerland. stephan.zeiter@aofoundation.org
FAU - Lezuo, Patrick
AU  - Lezuo P
FAU - Ito, Keita
AU  - Ito K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Biorheology
JT  - Biorheology
JID - 0372526
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 2/*pharmacology
MH  - Cattle
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Chondrogenesis/drug effects/physiology
MH  - Mechanotransduction, Cellular
MH  - Mesenchymal Stem Cells/drug effects/*physiology
MH  - Statistics, Nonparametric
MH  - Stress, Mechanical
MH  - Tissue Engineering/methods
MH  - Transforming Growth Factor beta1/*pharmacology
EDAT- 2009/03/03 09:00
MHDA- 2009/07/14 09:00
CRDT- 2009/03/03 09:00
PHST- 2009/03/03 09:00 [entrez]
PHST- 2009/03/03 09:00 [pubmed]
PHST- 2009/07/14 09:00 [medline]
AID - A3J74X4053G485H6 [pii]
AID - 10.3233/BIR-2009-0520 [doi]
PST - ppublish
SO  - Biorheology. 2009;46(1):45-55. doi: 10.3233/BIR-2009-0520.