PMID- 19253550
OWN - NLM
STAT- MEDLINE
DCOM- 20100617
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 26
IP  - 6
DP  - 2008 Nov
TI  - [Determination of eleven steroid hormones in animal muscle tissues and eggs using 
      ultra-performance liquid chromatography-tandem mass spectrometry].
PG  - 714-9
AB  - A credible method was developed for the simultaneous determination of eleven 
      steroid hormone residues in animal muscle tissues and eggs based on 
      ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). 
      The eleven steroid hormones were testosterone, methyltestosterone, trenbolone, 
      boldenone, nandrolone, methandienone, stanozolol, progesterone, nadrolone 
      propionate, testosterone propionate and nadrolone phenylpropionate. The samples 
      were extracted with tert-butyl methyl ether at alkaline pH and then cleaned up by 
      freezing-lipid filtration. All these drugs can be assayed in 10 min by UPLC-MS/MS 
      using electrospray ionization in positive ion mode and multiple reaction 
      monitoring mode. The limits of detection were 0.3 microg/kg for testosterone, 
      methyltestosterone, boldenone, methandienone and stanozolol, and 0.4 microg/kg 
      for trenbolone nandrolone, progesterone, testosterone propionate and nadrolone 
      phenylpropionate. Overall recoveries of testosterone, methyltestosterone, 
      boldenone, methandienone and stanozolol were 62.3% - 105% from pork, beef, mutton 
      and chicken muscle tissues, and eggs fortified at the 1, 2 and 10 microg/kg 
      levels, and the relative standard deviations (RSDs) were 0.5% - 15%. The 
      recoveries of trenbolone nandrolone, progesterone, testosterone propionate and 
      nadrolone phenylpropionate were higher than 50.0%, and the RSDs were lower than 
      16%. The matrix calibration curve for each drug was linear (r > 0.99) from 1 to 
      100 microg/L. The established method is simple, rapid, sensitive and specific, 
      and is appropriate for the identification and quantification of anabolic 
      androgenic steroids in animal muscle tissues and eggs.
FAU - He, Limin
AU  - He L
AD  - Department of Pharmacology and Toxicology, College of Veterinary Medicine, South 
      China Agriculture University, Guangzhou 510642, China. liminokhe@scau.edu.cn
FAU - Huang, Xianhui
AU  - Huang X
FAU - Fang, Binghu
AU  - Fang B
FAU - Huang, Shixin
AU  - Huang S
FAU - Cao, Ying
AU  - Cao Y
FAU - Chen, Jianxin
AU  - Chen J
FAU - Zeng, Zhenling
AU  - Zeng Z
FAU - Chen, Zhangliu
AU  - Chen Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Hormones)
RN  - 0 (Steroids)
SB  - IM
MH  - Analytic Sample Preparation Methods
MH  - Animals
MH  - Cattle
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Eggs/*analysis
MH  - Food Analysis
MH  - Food Contamination/*analysis
MH  - Hormones/*analysis/isolation & purification
MH  - Limit of Detection
MH  - Linear Models
MH  - Meat/analysis
MH  - Muscles/chemistry/*cytology
MH  - Steroids/*analysis/isolation & purification
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2009/03/04 09:00
MHDA- 2010/06/18 06:00
CRDT- 2009/03/04 09:00
PHST- 2009/03/04 09:00 [entrez]
PHST- 2009/03/04 09:00 [pubmed]
PHST- 2010/06/18 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2008 Nov;26(6):714-9.