PMID- 19255903
OWN - NLM
STAT- MEDLINE
DCOM- 20100920
LR  - 20131213
IS  - 1460-2709 (Electronic)
IS  - 1369-3786 (Linking)
VI  - 48
IP  - 1
DP  - 2010 Feb
TI  - A chronic chromoblastomycosis model by Fonsecaea monophora in Wistar rat.
PG  - 201-6
LID - 10.3109/13693780902785320 [doi]
AB  - Chromoblastomycosis is a chronic, cutaneous and subcutaneous infection 
      characterized by verrucose lesions. It is primarily caused by Fonsecaea monophora 
      in southern China and responds poorly to available therapies. In order to 
      investigate the pathogenicity of Fonsecaea monophora, we established a chronic 
      chromoblastomycosis animal model in Wistar rats. Suspensions of 2x10(6) cfu 
      conidia and hyphal fragments were intracutaneous (ic) and subcutaneous (sc) on 
      either side of the backs of the rats. Small nodules were formed at the 
      inoculation sites within the first week after inoculation. By the second week, 
      the nodules had enlarged and become soft, and pus could be aspirated from them. 
      In the fourth week, the nodules in the ic inoculated group had ulcerated and 
      sclerotic bodies were observed in smears prepared from all animals. In the 3rd 
      month after inoculation, the nodules at the sites of the ic inoculation had 
      become flat, with a thin black crust on the surface. For the ic inoculated group, 
      sequential biopsy studies revealed extensive necrosis with neutrophil 
      infiltration and sclerotic bodies and some debris of fungi in the 1st month, 
      sclerotic bodies inside multinucleated giant cells by the 2nd month and 
      widespread granulomatous inflammations in the 3rd month.
FAU - Xie, Zhi
AU  - Xie Z
AD  - Department of Dermatology, the Second Affiliated Hospital, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Zhang, Junming
AU  - Zhang J
FAU - Xi, Liyan
AU  - Xi L
FAU - Li, Xiqing
AU  - Li X
FAU - Wang, Liangchun
AU  - Wang L
FAU - Lu, Changming
AU  - Lu C
FAU - Sun, Jiufeng
AU  - Sun J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Med Mycol
JT  - Medical mycology
JID - 9815835
SB  - IM
MH  - Animals
MH  - Ascomycota/*pathogenicity
MH  - Chromoblastomycosis/*microbiology/*pathology
MH  - *Disease Models, Animal
MH  - Female
MH  - Histocytochemistry
MH  - Microscopy
MH  - Rats
MH  - Rats, Wistar
MH  - Skin/pathology
EDAT- 2009/03/04 09:00
MHDA- 2010/09/21 06:00
CRDT- 2009/03/04 09:00
PHST- 2009/03/04 09:00 [entrez]
PHST- 2009/03/04 09:00 [pubmed]
PHST- 2010/09/21 06:00 [medline]
AID - 909167937 [pii]
AID - 10.3109/13693780902785320 [doi]
PST - ppublish
SO  - Med Mycol. 2010 Feb;48(1):201-6. doi: 10.3109/13693780902785320.