PMID- 19258322
OWN - NLM
STAT- MEDLINE
DCOM- 20090608
LR  - 20211020
IS  - 0021-9258 (Print)
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Linking)
VI  - 284
IP  - 18
DP  - 2009 May 1
TI  - Intersectin regulates dendritic spine development and somatodendritic endocytosis 
      but not synaptic vesicle recycling in hippocampal neurons.
PG  - 12410-9
LID - 10.1074/jbc.M809746200 [doi]
AB  - Intersectin-short (intersectin-s) is a multimodule scaffolding protein 
      functioning in constitutive and regulated forms of endocytosis in non-neuronal 
      cells and in synaptic vesicle (SV) recycling at the neuromuscular junction of 
      Drosophila and Caenorhabditis elegans. In vertebrates, alternative splicing 
      generates a second isoform, intersectin-long (intersectin-l), that contains 
      additional modular domains providing a guanine nucleotide exchange factor 
      activity for Cdc42. In mammals, intersectin-s is expressed in multiple tissues 
      and cells, including glia, but excluded from neurons, whereas intersectin-l is a 
      neuron-specific isoform. Thus, intersectin-I may regulate multiple forms of 
      endocytosis in mammalian neurons, including SV endocytosis. We now report, 
      however, that intersectin-l is localized to somatodendritic regions of cultured 
      hippocampal neurons, with some juxtanuclear accumulation, but is excluded from 
      synaptophysin-labeled axon terminals. Consistently, intersectin-l knockdown (KD) 
      does not affect SV recycling. Instead intersectin-l co-localizes with clathrin 
      heavy chain and adaptor protein 2 in the somatodendritic region of neurons, and 
      its KD reduces the rate of transferrin endocytosis. The protein also co-localizes 
      with F-actin at dendritic spines, and intersectin-l KD disrupts spine maturation 
      during development. Our data indicate that intersectin-l is indeed an important 
      regulator of constitutive endocytosis and neuronal development but that it is not 
      a prominent player in the regulated endocytosis of SVs.
FAU - Thomas, Sebastien
AU  - Thomas S
AD  - Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill 
      University, Montreal, Quebec H3A 2B4, Canada.
FAU - Ritter, Brigitte
AU  - Ritter B
FAU - Verbich, David
AU  - Verbich D
FAU - Sanson, Claire
AU  - Sanson C
FAU - Bourbonniere, Lyne
AU  - Bourbonniere L
FAU - McKinney, R Anne
AU  - McKinney RA
FAU - McPherson, Peter S
AU  - McPherson PS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090303
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Actins)
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Protein Isoforms)
RN  - 0 (intersectin 1)
RN  - 114899-12-6 (Clathrin Heavy Chains)
SB  - IM
MH  - Actins/genetics/metabolism
MH  - Adaptor Proteins, Vesicular Transport/genetics/*metabolism
MH  - Alternative Splicing/physiology
MH  - Animals
MH  - Axons/metabolism
MH  - Caenorhabditis elegans
MH  - Clathrin Heavy Chains/genetics/metabolism
MH  - Dendritic Spines/*metabolism
MH  - Drosophila melanogaster
MH  - Endocytosis/*physiology
MH  - Gene Knockdown Techniques
MH  - Hippocampus/cytology/*metabolism
MH  - Protein Isoforms/genetics/metabolism
MH  - Protein Structure, Tertiary/physiology
MH  - Rats
MH  - Synaptic Vesicles/genetics/*metabolism
PMC - PMC2673308
EDAT- 2009/03/05 09:00
MHDA- 2009/06/09 09:00
PMCR- 2010/05/01
CRDT- 2009/03/05 09:00
PHST- 2009/03/05 09:00 [entrez]
PHST- 2009/03/05 09:00 [pubmed]
PHST- 2009/06/09 09:00 [medline]
PHST- 2010/05/01 00:00 [pmc-release]
AID - S0021-9258(20)58399-3 [pii]
AID - 12410 [pii]
AID - 10.1074/jbc.M809746200 [doi]
PST - ppublish
SO  - J Biol Chem. 2009 May 1;284(18):12410-9. doi: 10.1074/jbc.M809746200. Epub 2009 
      Mar 3.