PMID- 19259271
OWN - NLM
STAT- MEDLINE
DCOM- 20090427
LR  - 20220409
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 4
IP  - 3
DP  - 2009
TI  - MicroRNA expression in human omental and subcutaneous adipose tissue.
PG  - e4699
LID - 10.1371/journal.pone.0004699 [doi]
LID - e4699
AB  - MicroRNAs (miRNAs) are small non-coding RNAs, that play important regulatory 
      roles in a variety of biological processes, including development, 
      differentiation, apoptosis, and metabolism. In mammals, miRNAs have been shown to 
      modulate adipocyte differentiation. Therefore, we performed a global miRNA gene 
      expression assay in different fat depots of overweight and obese individuals to 
      investigate whether miRNA expression in human adipose tissue is fat-depot 
      specific and associated with parameters of obesity and glucose metabolism. Paired 
      samples of abdominal subcutaneous (SC) and intraabdominal omental adipose tissue 
      were obtained from fifteen individuals with either normal glucose tolerance (NGT, 
      n = 9) or newly diagnosed type 2 diabetes (T2D, n = 6). Expression of 155 miRNAs 
      was carried out using the TaqMan(R)MicroRNA Assays Human Panel Early Access Kit 
      (Applied Biosystems, Darmstadt, Germany). We identified expression of 106 (68%) 
      miRNAs in human omental and SC adipose tissue. There was no miRNA exclusively 
      expressed in either fat depot, suggesting common developmental origin of both fat 
      depots. Sixteen miRNAs (4 in NGT, 12 in T2D group) showed a significant fat depot 
      specific expression pattern. We identified significant correlations between the 
      expression of miRNA-17-5p, -132, -99a, -134, 181a, -145, -197 and both adipose 
      tissue morphology and key metabolic parameters, including visceral fat area, 
      HbA(1c), fasting plasma glucose, and circulating leptin, adiponectin, 
      interleukin-6. In conclusion, microRNA expression differences may contribute to 
      intrinsic differences between omental and subcutaneous adipose tissue. In 
      addition, human adipose tissue miRNA expression correlates with adipocyte 
      phenotype, parameters of obesity and glucose metabolism.
FAU - Kloting, Nora
AU  - Kloting N
AD  - Department of Medicine, University of Leipzig, Leipzig, Germany.
FAU - Berthold, Susan
AU  - Berthold S
FAU - Kovacs, Peter
AU  - Kovacs P
FAU - Schon, Michael R
AU  - Schon MR
FAU - Fasshauer, Mathias
AU  - Fasshauer M
FAU - Ruschke, Karen
AU  - Ruschke K
FAU - Stumvoll, Michael
AU  - Stumvoll M
FAU - Bluher, Matthias
AU  - Bluher M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090304
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (MicroRNAs)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Abdominal Fat/*metabolism
MH  - Aged
MH  - Gene Expression
MH  - Glucose/metabolism
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Obesity/genetics/metabolism
MH  - Omentum/*metabolism
MH  - Subcutaneous Fat/*metabolism
PMC - PMC2649537
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2009/03/05 09:00
MHDA- 2009/04/28 09:00
PMCR- 2009/03/04
CRDT- 2009/03/05 09:00
PHST- 2008/12/08 00:00 [received]
PHST- 2009/01/14 00:00 [accepted]
PHST- 2009/03/05 09:00 [entrez]
PHST- 2009/03/05 09:00 [pubmed]
PHST- 2009/04/28 09:00 [medline]
PHST- 2009/03/04 00:00 [pmc-release]
AID - 08-PONE-RA-07649R1 [pii]
AID - 10.1371/journal.pone.0004699 [doi]
PST - ppublish
SO  - PLoS One. 2009;4(3):e4699. doi: 10.1371/journal.pone.0004699. Epub 2009 Mar 4.