PMID- 19261778
OWN - NLM
STAT- MEDLINE
DCOM- 20090623
LR  - 20240416
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 16
IP  - 5
DP  - 2009 May
TI  - Antibody response to porcine reproductive and respiratory syndrome virus (PRRSV) 
      nonstructural proteins and implications for diagnostic detection and 
      differentiation of PRRSV types I and II.
PG  - 628-35
LID - 10.1128/CVI.00483-08 [doi]
AB  - To further characterize the humoral immune response of pigs to porcine 
      reproductive and respiratory syndrome virus (PRRSV), direct enzyme-linked 
      immunosorbent assays (ELISA) were used to study the kinetics of antibody 
      responses directed against PRRSV nonstructural proteins in pigs experimentally 
      exposed to the virus. The highest immunoreactivities were against nsp1, nsp2, and 
      nsp7. Using the recombinant nsp7 as an antigen, we validated a dual ELISA for the 
      simultaneous detection and differentiation of serum antibodies against type I and 
      type II PRRSV. Receiver operating characteristic analysis based on 1,334 
      known-positive and 1,357 known-negative samples showed good specificity (98.3% to 
      type I and 99.3% to type II) and sensitivity (97.4% for type I and 99.8% for type 
      II). To differentiate type I and type II PRRSV, 470 sera originating from 
      experimentally inoculated pigs were tested, and positive sera were correctly 
      differentiated in 469 of 470 samples. The capability of the nsp7 dual ELISA to 
      detect serum antibody responses from pigs infected with various genetically 
      different field strains was determined. The nsp7 dual ELISA possessed 97.6% 
      agreement with the Idexx HerdChek PRRS 2XR ELISA. In further testing of Idexx 
      ELISA suspected false-positive samples, the nsp7 dual ELISA resolved 98% of the 
      samples as negative. Taken together, these results indicate that the nsp7 dual 
      ELISA can be used as a differential test for PRRSV serology with high levels of 
      sensitivity and specificity. This ELISA offers an additional tool for routine or 
      follow-up diagnostics, as well as having substantial value in epidemiological 
      surveys and outbreak investigations.
FAU - Brown, Elizabeth
AU  - Brown E
AD  - Department of Veterinary Science, Center for Infectious Disease Research and 
      Vaccinology, South Dakota State University, Brookings, SD 57007-1396, USA.
FAU - Lawson, Steven
AU  - Lawson S
FAU - Welbon, Craig
AU  - Welbon C
FAU - Gnanandarajah, Josephine
AU  - Gnanandarajah J
FAU - Li, Juan
AU  - Li J
FAU - Murtaugh, Michael P
AU  - Murtaugh MP
FAU - Nelson, Eric A
AU  - Nelson EA
FAU - Molina, Ramon M
AU  - Molina RM
FAU - Zimmerman, Jeffery J
AU  - Zimmerman JJ
FAU - Rowland, Raymond R R
AU  - Rowland RR
FAU - Fang, Ying
AU  - Fang Y
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090304
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*classification/*immunology
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Viral Nonstructural Proteins/*immunology
PMC - PMC2681581
EDAT- 2009/03/06 09:00
MHDA- 2009/06/24 09:00
PMCR- 2009/11/01
CRDT- 2009/03/06 09:00
PHST- 2009/03/06 09:00 [entrez]
PHST- 2009/03/06 09:00 [pubmed]
PHST- 2009/06/24 09:00 [medline]
PHST- 2009/11/01 00:00 [pmc-release]
AID - CVI.00483-08 [pii]
AID - 0483-08 [pii]
AID - 10.1128/CVI.00483-08 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2009 May;16(5):628-35. doi: 10.1128/CVI.00483-08. Epub 2009 
      Mar 4.