PMID- 19261910
OWN - NLM
STAT- MEDLINE
DCOM- 20090427
LR  - 20211020
IS  - 0363-6143 (Print)
IS  - 1522-1563 (Electronic)
IS  - 0363-6143 (Linking)
VI  - 296
IP  - 3
DP  - 2009 Mar
TI  - Directed differentiation of mouse cochlear neural progenitors in vitro.
PG  - C441-52
LID - 10.1152/ajpcell.00324.2008 [doi]
AB  - Multipotent cochlear neural progenitors (CNPs) in the organ of Corti hold the 
      promise for cell replacement in degenerative hearing disorders. However, not much 
      is known about the CNPs and the specific conditions for their differentiation. 
      Here we isolate the CNPs from the postnatal day 1 organ of Corti in mice and 
      demonstrate their capability to self-renew and to differentiate into hair 
      cell-like and neuronal cell-like phenotypes under the guidance of sonic hedgehog 
      (SHH), epidermal growth factor (EGF), retinoic acid (RA), and brain-derived 
      neurotrophic factor (BDNF), herein termed SERB (abbreviation of SHH, EGF, RA, and 
      BDNF) in an asymmetric or symmetric manner from clonal isolates. Differentiation 
      of CNPs into hair cells by SERB was dependent on the ERK signaling pathway, 
      whereas the differentiation of CNPs into neurons by SERB was not. This work 
      develops a new in vitro methodology for the maintenance and self-regeneration of 
      CNPs for future design of regenerative strategies for hearing disorders.
FAU - Lin, Jizhen
AU  - Lin J
AD  - Department of Otolaryngology, 216 Lions Research Bldg., Univ. of Minnesota, 2001 
      Sixth St. S.E., Minneapolis, MN 55455, USA. linxx004@tc.umn.edu
FAU - Feng, Ling
AU  - Feng L
FAU - Hamajima, Yuki
AU  - Hamajima Y
FAU - Komori, Masahiro
AU  - Komori M
FAU - Burns, Terry C
AU  - Burns TC
FAU - Fukudome, Shinji
AU  - Fukudome S
FAU - Anderson, John
AU  - Anderson J
FAU - Wang, Dong
AU  - Wang D
FAU - Verfaillie, Catherine M
AU  - Verfaillie CM
FAU - Low, Walter C
AU  - Low WC
LA  - eng
GR  - R01 DC008165-02/DC/NIDCD NIH HHS/United States
GR  - R03 CA107989/CA/NCI NIH HHS/United States
GR  - P30 CD-04660/CD/ODCDC CDC HHS/United States
GR  - R01 DC008165/DC/NIDCD NIH HHS/United States
GR  - R01 DC-008165/DC/NIDCD NIH HHS/United States
GR  - R03 CA-107989/CA/NCI NIH HHS/United States
GR  - R03 CA107989-02/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20081217
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 5688UTC01R (Tretinoin)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
RIN - Am J Physiol Cell Physiol. 2009 Dec;297(6):C1596. PMID: 19948690
MH  - Animals
MH  - Animals, Newborn
MH  - Biomarkers/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Culture Techniques
MH  - *Cell Differentiation/drug effects
MH  - *Cell Proliferation/drug effects
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Epidermal Growth Factor/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors/metabolism
MH  - Hair Cells, Auditory/drug effects/metabolism/*physiology
MH  - Hedgehog Proteins/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons/drug effects/metabolism/*physiology
MH  - Organ of Corti/cytology/drug effects/metabolism/*physiology
MH  - Phenotype
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Signal Transduction
MH  - Stem Cells/drug effects/metabolism/*physiology
MH  - Time Factors
MH  - Tretinoin/metabolism
PMC - PMC2660259
EDAT- 2009/03/06 09:00
MHDA- 2009/04/28 09:00
PMCR- 2010/03/01
CRDT- 2009/03/06 09:00
PHST- 2009/03/06 09:00 [entrez]
PHST- 2009/03/06 09:00 [pubmed]
PHST- 2009/04/28 09:00 [medline]
PHST- 2010/03/01 00:00 [pmc-release]
AID - 00324.2008 [pii]
AID - 10.1152/ajpcell.00324.2008 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2009 Mar;296(3):C441-52. doi: 
      10.1152/ajpcell.00324.2008. Epub 2008 Dec 17.