PMID- 19262251
OWN - NLM
STAT- MEDLINE
DCOM- 20090508
LR  - 20151119
IS  - 1536-3678 (Electronic)
IS  - 1077-4114 (Linking)
VI  - 31
IP  - 3
DP  - 2009 Mar
TI  - Expression of high mobility group A2 protein in retinoblastoma and its 
      association with clinicopathologic features.
PG  - 209-14
LID - 10.1097/MPH.0b013e318197978d [doi]
AB  - Retinoblastoma (RB) is the commonest primary intraocular tumor in children. 
      Overexpression of the high mobility group (HMG) A2 protein has been observed in a 
      variety of malignant tumors and often correlates with poor prognosis. We studied 
      the expression of HMGA2 in primary tumor samples and correlated with 
      clinicopathologic features such as invasion, differentiation, and laterality of 
      the tumors. Among 64 tumors, there were 29 tumors with invasion of the optic 
      nerve, choroid, and/or orbit and 35 tumors without invasion. HMGA2 
      immunoreactivity was evaluated on archival paraffin sections and the results 
      confirmed by Western blotting on 12 fresh tumor samples. Among 29 tumors with 
      invasion, HMGA2 was strongly positive (++) in 10 tumors, moderately positive (+) 
      in 11 tumors. Among 35 tumors without invasion, HMGA2 was strongly positive (++) 
      in 6 tumors, moderately positive (+) in 6 tumors. Tumors with invasion showed 
      significantly higher expression of HMGA2 compared with tumors without invasion 
      (P<0.01). Non-neoplastic retina was negative for HMGA2. There was no correlation 
      between HMGA2 expression and differentiation/laterality. Western blotting 
      revealed that 7 tumors were strongly positive, 2 were moderately positive, and 1 
      was faintly positive for HMGA2. Our study has demonstrated the HMGA2 expression 
      in a large cohort of primary retinoblastoma tumors and its correlation with 
      invasiveness.
FAU - Venkatesan, Nalini
AU  - Venkatesan N
AD  - L and T Department of Ocular Pathology, Vision Research Foundation, Sankara 
      Nethralaya, Chennai, India.
FAU - Kandalam, Mallikarjuna
AU  - Kandalam M
FAU - Pasricha, Gunisha
AU  - Pasricha G
FAU - Sumantran, Venil
AU  - Sumantran V
FAU - Manfioletti, Guidalberto
AU  - Manfioletti G
FAU - Ono, Santa Jeremy
AU  - Ono SJ
FAU - Reddy, Maddy Ashwin
AU  - Reddy MA
FAU - Krishnakumar, Subramanian
AU  - Krishnakumar S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Hematol Oncol
JT  - Journal of pediatric hematology/oncology
JID - 9505928
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HMGA2 Protein)
SB  - IM
MH  - Biomarkers, Tumor/analysis
MH  - Blotting, Western
MH  - Child, Preschool
MH  - Female
MH  - HMGA2 Protein/*biosynthesis
MH  - Humans
MH  - Immunohistochemistry
MH  - Infant
MH  - Male
MH  - Neoplasm Invasiveness
MH  - Prognosis
MH  - Retinal Neoplasms/*metabolism/*pathology
MH  - Retinoblastoma/*metabolism/*pathology
EDAT- 2009/03/06 09:00
MHDA- 2009/05/09 09:00
CRDT- 2009/03/06 09:00
PHST- 2009/03/06 09:00 [entrez]
PHST- 2009/03/06 09:00 [pubmed]
PHST- 2009/05/09 09:00 [medline]
AID - 00043426-200903000-00014 [pii]
AID - 10.1097/MPH.0b013e318197978d [doi]
PST - ppublish
SO  - J Pediatr Hematol Oncol. 2009 Mar;31(3):209-14. doi: 
      10.1097/MPH.0b013e318197978d.