PMID- 19262252
OWN - NLM
STAT- MEDLINE
DCOM- 20090508
LR  - 20161125
IS  - 1536-3678 (Electronic)
IS  - 1077-4114 (Linking)
VI  - 31
IP  - 3
DP  - 2009 Mar
TI  - 8q deletion in MYCN-amplified neuroblastoma of a child born from assisted 
      reproductive technology.
PG  - 215-9
LID - 10.1097/MPH.0b013e3181979c94 [doi]
AB  - The occurrence of pediatric cancer in children born from assisted reproductive 
      technologies has been sporadically reported. Chromosomal characterization of the 
      neoplasic disease in this setting is poorly described. In the present study, 
      neuroblastoma cells from a 13-month-old infant boy born after intracytoplasmatic 
      sperm injection were characterized by combining conventional cytogenetics, 
      fluorescence in situ hybridization (FISH), comparative genomic hybridization, and 
      quantitative polymerase chain reaction methods. Cytogenetic analysis of 
      neuroblastoma (NB) metaphase spreads at the time of diagnosis revealed numerous 
      centromere-free extrachromosomal double minutes, suggesting high MYCN 
      amplification. Comparative genomic hybridization analysis demonstrated the 
      amplification of 2q24 approximately pter, with additional gain of the long arm of 
      chromosome 17. Chromosome losses involved 8q, 9q, and 11q. No deletion of 1p was 
      found. MYCN amplification was confirmed by quantitative polymerase chain reaction 
      and fluorescence in situ hybridization analysis. This report describes several 
      chromosomal abnormalities that were present in NB of a child born after 
      intracytoplasmatic sperm injection. Besides some well described and prognostic 
      genetic findings in NB as MYCN amplification, gain on 17q and losses on 9q and 
      11q23, we report an unusual deletion involving 8q region in this disease. Whether 
      this genetic abnormality may be associated to assisted reproductive technologies 
      deserves further investigation.
FAU - Brassesco, Maria Sol
AU  - Brassesco MS
AD  - Division of Pediatric Oncology, Department of Pediatrics, Faculty of Medicine of 
      Ribeirao Preto, University of Sao Paulo, SP, Brazil. marsol@rge.fmrp.usp.br
FAU - Valera, Elvis Terci
AU  - Valera ET
FAU - de Oliveira, Fabio Morato
AU  - de Oliveira FM
FAU - de Paula Queiroz, Rosane Gomes
AU  - de Paula Queiroz RG
FAU - Scrideli, Carlos Alberto
AU  - Scrideli CA
FAU - Sakamoto-Hojo, Elza Tiemi
AU  - Sakamoto-Hojo ET
FAU - Tone, Luiz Gonzaga
AU  - Tone LG
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Hematol Oncol
JT  - Journal of pediatric hematology/oncology
JID - 9505928
RN  - 0 (MYCN protein, human)
RN  - 0 (N-Myc Proto-Oncogene Protein)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins)
SB  - IM
MH  - Abdominal Neoplasms/*genetics/pathology
MH  - Antineoplastic Combined Chemotherapy Protocols/therapeutic use
MH  - Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - N-Myc Proto-Oncogene Protein
MH  - Neuroblastoma/*genetics/pathology
MH  - Nuclear Proteins/*genetics
MH  - Oncogene Proteins/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sperm Injections, Intracytoplasmic/*adverse effects
EDAT- 2009/03/06 09:00
MHDA- 2009/05/09 09:00
CRDT- 2009/03/06 09:00
PHST- 2009/03/06 09:00 [entrez]
PHST- 2009/03/06 09:00 [pubmed]
PHST- 2009/05/09 09:00 [medline]
AID - 00043426-200903000-00015 [pii]
AID - 10.1097/MPH.0b013e3181979c94 [doi]
PST - ppublish
SO  - J Pediatr Hematol Oncol. 2009 Mar;31(3):215-9. doi: 10.1097/MPH.0b013e3181979c94.