PMID- 19262293
OWN - NLM
STAT- MEDLINE
DCOM- 20090430
LR  - 20211020
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 65
IP  - 2
DP  - 2009 Feb
TI  - Effect of probiotics Lactobacillus acidophilus on Citrobacter rodentium colitis: 
      the role of dendritic cells.
PG  - 169-75
LID - 10.1203/PDR.0b013e31818d5a06 [doi]
AB  - Modulation of the intestinal immune response early in life by administration of 
      probiotic bacteria may be an effective strategy for preventing or attenuating 
      infectious diarrhea. We preinoculated the mice early in life with the probiotic 
      bacteria Lactobacillus acidophilus NCFM (La) at age 2 wk. Dendritic cells (DCs) 
      were collected and purified from mesenteric lymph nodes (MLN) and spleens of the 
      BalbC/ByJ mice. DC isolation and adoptive transfer was used to examine the 
      function of probiotics. We demonstrated that when mice were adoptively 
      transferred with La-primed DCs (t-LaDC) instead of oral consumption with La, 
      there was a similar effect on fecal bacteria counts, IgA levels, and colonic 
      histopathology, as well as cytokine levels in MLN when there was intestinal 
      bacterial infection. The above findings suggest that DCs play a key role in 
      probiotics attenuating Citrobacter rodentium (Cr) colitis. Moreover, the location 
      of La-primed DC hints that there is interaction of DCs and T cells in the 
      digestive system of the host. Up-regulated expression of a surface marker on DCs 
      indicated that inoculation with probiotics will stimulate the function of DCs, 
      thereby further increasing immune response triggered by DC.
FAU - Chen, Chien-Chang
AU  - Chen CC
AD  - Department of Pediatrics, Chang Gung Memorial Hospital, Chang Gung Children's 
      Hospital, Chang Gung University College of Medicine, Taoyuan, 333, Taiwan.
FAU - Chiu, Cheng-Hsun
AU  - Chiu CH
FAU - Lin, Tzou-Yien
AU  - Lin TY
FAU - Shi, Hai Ning
AU  - Shi HN
FAU - Walker, W Allan
AU  - Walker WA
LA  - eng
GR  - P30 DK040561/DK/NIDDK NIH HHS/United States
GR  - P30 DK040561-13/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Antigens, Bacterial)
RN  - 0 (CD11c Antigen)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin A)
SB  - IM
MH  - Administration, Oral
MH  - Adoptive Transfer
MH  - Animals
MH  - Antigens, Bacterial/immunology
MH  - CD11c Antigen/metabolism
MH  - Cells, Cultured
MH  - Citrobacter rodentium/*pathogenicity
MH  - Colitis/immunology/microbiology/*prevention & control
MH  - Colon/immunology/*microbiology/pathology
MH  - Cytokines/metabolism
MH  - Dendritic Cells/immunology/*microbiology
MH  - Enterobacteriaceae Infections/immunology/microbiology/*prevention & control
MH  - Feces/microbiology
MH  - Female
MH  - Immunoglobulin A/metabolism
MH  - Lactobacillus acidophilus/growth & development/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Probiotics/*administration & dosage
MH  - T-Lymphocytes/immunology/microbiology
MH  - Time Factors
PMC - PMC3206789
MID - NIHMS330512
EDAT- 2009/03/06 09:00
MHDA- 2009/05/01 09:00
PMCR- 2011/11/02
CRDT- 2009/03/06 09:00
PHST- 2009/03/06 09:00 [entrez]
PHST- 2009/03/06 09:00 [pubmed]
PHST- 2009/05/01 09:00 [medline]
PHST- 2011/11/02 00:00 [pmc-release]
AID - 00006450-200902000-00008 [pii]
AID - 10.1203/PDR.0b013e31818d5a06 [doi]
PST - ppublish
SO  - Pediatr Res. 2009 Feb;65(2):169-75. doi: 10.1203/PDR.0b013e31818d5a06.