PMID- 19264231
OWN - NLM
STAT- MEDLINE
DCOM- 20090402
LR  - 20171116
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 190
IP  - 1
DP  - 2009 Apr 1
TI  - Frequency of 5'IGH deletions in B-cell chronic lymphocytic leukemia.
PG  - 33-9
LID - 10.1016/j.cancergencyto.2008.12.004 [doi]
AB  - In a retrospective analysis of a large group of cases (n=291) with B-cell CLL 
      diagnosis, the various characteristics of IGH aberrations as identified by 
      fluorescence in situ hybridization (FISH) probes were studied. Conventional 
      cytogenetic and FISH studies with the standard panel (13q14, 11q13, 17p13, 12 
      centromere), and with IGH break-apart probes were done for each case. Abnormal 
      karyotypes were detected with conventional cytogenetics in 29% of cases, and FISH 
      detected abnormalities in 70%. Deletion of 13q14 was the most frequent anomaly, 
      followed by trisomy 12, deletion of 11q, and deletion of 17p. Among the IGH 
      abnormalities detected, translocations with unknown partners (split signals) 
      occurred in only a small group of patients (15%). Instead, deletion of 5'IGH, 
      corresponding to the variable IGH segment (IGH(V)) was the most recurrent 
      aberration, observed in 82% (the second most common finding among our patients). 
      This deletion was associated with good prognostic markers: 13q14 deletion, normal 
      karyotype, and CD38 and ZAP-70 negative expression. Although not exclusive to 
      CLL, the deletion occurred in a high frequency, in contrast to its rarity in 
      other B-cell lymphoproliferative disorders. Longitudinal studies are warranted, 
      to determine when in the disease progression this abnormality is acquired, as a 
      potential early marker, and its impact on the natural history of CLL.
FAU - Quintero-Rivera, Fabiola
AU  - Quintero-Rivera F
AD  - UCLA Clinical and Molecular Cytogenetics Laboratory, Department of Pathology & 
      Laboratory Medicine, David Geffen School of Medicine, University of California at 
      Los Angeles, 1000 Veteran Avenue, Los Angeles, CA 90024, USA. 
      fquintero@mednet.ucla.edu
FAU - Nooraie, Farzad
AU  - Nooraie F
FAU - Rao, P Nagesh
AU  - Rao PN
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - EC 2.7.10.2 (ZAP-70 Protein-Tyrosine Kinase)
RN  - EC 3.2.2.6 (ADP-ribosyl Cyclase 1)
SB  - IM
MH  - 5' Flanking Region/*genetics
MH  - ADP-ribosyl Cyclase 1/genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Y
MH  - Female
MH  - *Gene Deletion
MH  - Gene Expression Regulation, Leukemic
MH  - Gene Frequency
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - ZAP-70 Protein-Tyrosine Kinase/genetics
EDAT- 2009/03/07 09:00
MHDA- 2009/04/03 09:00
CRDT- 2009/03/07 09:00
PHST- 2008/10/24 00:00 [received]
PHST- 2008/11/21 00:00 [accepted]
PHST- 2009/03/07 09:00 [entrez]
PHST- 2009/03/07 09:00 [pubmed]
PHST- 2009/04/03 09:00 [medline]
AID - S0165-4608(08)00705-X [pii]
AID - 10.1016/j.cancergencyto.2008.12.004 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2009 Apr 1;190(1):33-9. doi: 
      10.1016/j.cancergencyto.2008.12.004.