PMID- 1926576 OWN - NLM STAT- MEDLINE DCOM- 19911031 LR - 20131121 IS - 0041-3771 (Print) IS - 0041-3771 (Linking) VI - 33 IP - 2 DP - 1991 TI - [An evaluation of the conformational changes in the superhelical DNA of eukaryotic cells by direct nucleoid fluorometry. II. The characteristics of the change in acridine orange fluorescence in studying the superhelical DNA of rat thymocytes]. PG - 89-93 AB - Comparative studies of acridine orange (AO) and ethidium bromide interactions with supercoiled DNA (scDNA) of thymocytes were performed in which various conformational changes were induced. AO may be efficiently used for evaluation of conformational alterations of scDNA. Moreover, employing the maximum values of AO fluorescence allows to determine the maximum levels of scDNA relaxation. FAU - Reshchikov, A M AU - Reshchikov AM FAU - Vashchenko, V I AU - Vashchenko VI FAU - Komar, V E AU - Komar VE LA - rus PT - Comparative Study PT - English Abstract PT - Journal Article TT - Otsenka konformatsionnykh izmenenii superspiral'noi DNK eukarioticheskikh kletok metodom priamoi fluorimetrii nukleoidov. II. Osobennosti izmeneniia fluorestsentsii akridinovogo oranzhevogo pri izuchenii superspiral'noi DNK timotsitov krys. PL - Russia (Federation) TA - Tsitologiia JT - Tsitologiia JID - 0417363 RN - 0 (DNA, Superhelical) RN - 0 (Intercalating Agents) RN - EN464416SI (Ethidium) RN - F30N4O6XVV (Acridine Orange) SB - IM MH - Acridine Orange MH - Animals MH - Cell Nucleus/*ultrastructure MH - DNA, Superhelical/*ultrastructure MH - Ethidium MH - Flow Cytometry MH - Fluorescence MH - Intercalating Agents MH - Lymphocytes/ultrastructure MH - *Nucleic Acid Conformation MH - Rats MH - Thymus Gland/*ultrastructure MH - Viscosity EDAT- 1991/01/01 00:00 MHDA- 1991/01/01 00:01 CRDT- 1991/01/01 00:00 PHST- 1991/01/01 00:00 [pubmed] PHST- 1991/01/01 00:01 [medline] PHST- 1991/01/01 00:00 [entrez] PST - ppublish SO - Tsitologiia. 1991;33(2):89-93.