PMID- 19267421 OWN - NLM STAT- MEDLINE DCOM- 20090814 LR - 20151119 IS - 1097-4547 (Electronic) IS - 0360-4012 (Linking) VI - 87 IP - 9 DP - 2009 Jul TI - BDNF and extracellular matrix regulate differentiation of mice neurosphere-derived cells into a GABAergic neuronal phenotype. PG - 1986-96 LID - 10.1002/jnr.22041 [doi] AB - Differentiation of neurosphere-derived cells is regulated by extracellular cues, namely, growth factors and proteins of the extracellular matrix (ECM). In this study we analyzed the influence of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), retinoic acid plus potassium chloride (RA-KCl), and the nonsynthetic ECMs laminin (LN) and fibronectin (FN) versus the synthetic adhesion substrate poly-L-lysine (PLL) in the in vitro differentiation of postnatal neurosphere cells. BDNF increased the number of differentiated neurons and decreased the number of neuronal precursors (nestin-positive cells) compared with NGF or RA-KCl. Moreover, cells treated with BDNF plus B27 supplement acquired a gamma-aminobutyric acid (GABA)-ergic phenotype and showed increased survival. No significant differences were found in the number of differentiated neurons in the presence of the ECMs alone. Nevertheless, FN or PLL in combination with BDNF promoted the acquisition of a GABAergic phenotype. The results obtained in this study highlight the importance of growth factors and ECM proteins for the potential of neurosphere cells to differentiate into neurons. CI - (c) 2009 Wiley-Liss, Inc. FAU - Silva, Ana AU - Silva A AD - Center for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, Coimbra, Portugal. FAU - Pereira, Jorge AU - Pereira J FAU - Oliveira, Catarina R AU - Oliveira CR FAU - Relvas, Joao Bettencourt AU - Relvas JB FAU - Rego, A Cristina AU - Rego AC LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Neurosci Res JT - Journal of neuroscience research JID - 7600111 RN - 0 (Biomarkers) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Extracellular Matrix Proteins) RN - 0 (Fibronectins) RN - 0 (Intermediate Filament Proteins) RN - 0 (Laminin) RN - 0 (Nerve Tissue Proteins) RN - 0 (Nes protein, mouse) RN - 0 (Nestin) RN - 25104-18-1 (Polylysine) RN - 56-12-2 (gamma-Aminobutyric Acid) RN - 5688UTC01R (Tretinoin) RN - 9061-61-4 (Nerve Growth Factor) SB - IM MH - Animals MH - Animals, Newborn MH - Biomarkers/analysis/metabolism MH - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology MH - Cell Differentiation/drug effects/physiology MH - Cell Proliferation/drug effects MH - Cell Survival/drug effects/physiology MH - Cells, Cultured MH - Extracellular Matrix Proteins/*metabolism/pharmacology MH - Fibronectins/metabolism/pharmacology MH - Intermediate Filament Proteins/analysis/metabolism MH - Laminin/metabolism/pharmacology MH - Mice MH - Nerve Growth Factor/metabolism/pharmacology MH - Nerve Tissue Proteins/analysis/metabolism MH - Nestin MH - Neurogenesis/drug effects/physiology MH - Neurons/cytology/drug effects/*metabolism MH - Phenotype MH - Polylysine/pharmacology MH - Spheroids, Cellular/cytology/drug effects/*metabolism MH - Stem Cells/cytology/drug effects/*metabolism MH - Tretinoin/metabolism/pharmacology MH - gamma-Aminobutyric Acid/*metabolism EDAT- 2009/03/10 09:00 MHDA- 2009/08/15 09:00 CRDT- 2009/03/10 09:00 PHST- 2009/03/10 09:00 [entrez] PHST- 2009/03/10 09:00 [pubmed] PHST- 2009/08/15 09:00 [medline] AID - 10.1002/jnr.22041 [doi] PST - ppublish SO - J Neurosci Res. 2009 Jul;87(9):1986-96. doi: 10.1002/jnr.22041.