PMID- 19269281
OWN - NLM
STAT- MEDLINE
DCOM- 20090729
LR  - 20090501
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1269
DP  - 2009 May 7
TI  - Alternative splicing variants and DNA methylation status of BDNF in inbred 
      chicken lines.
PG  - 1-10
LID - 10.1016/j.brainres.2009.01.071 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) has multiple alternative splicing 
      variants and plays diverse biological functions in mammals, including neuronal 
      survival, cholesterol metabolism, cell differentiation and tumor development. 
      However, genomic structures of chicken BDNF (cBDNF) variants and its potential 
      functions are still undefined. Here, we characterized two novel alternative 
      splicing variants of cBDNF, cBDNF1 and cBDNF2, via combining comparative genomics 
      methods and molecular techniques in inbred chicken line 6(3) and line 7(2), which 
      have been developed to be resistant and susceptible, respectively, to Marek's 
      disease tumor since 1939. Both cBDNFs consist of a bipartite transcript, with 
      different 5' exons, exon I (298 bp) in cBDNF1 and exon II (286 bp) in cBDNF2, 
      each of which is spliced to the common 3' exon IV. Exon I and IV are highly 
      conserved between chicken and mammals, whereas exon II is unique for chicken. The 
      amino acid sequence of cBDNF1 contains 8 additional amino acids in the N terminal 
      compared to cBDNF2. cBDNF1 and cBDNF2 were only expressed in the hypothalamus 
      among eight tissues, and cBDNF2 showed lower expression than that of cBDNF1 in 
      both lines. The expression level of cBDNF1 was significantly higher in line 7(2) 
      than in line 6(3) (P<0.01). Notably, the DNA methylation levels on the 
      cis-regulatory region of cBDNF1 was negatively correlated with its expression 
      level, which suggests that the mRNA expression level of cBDNF1 may be related to 
      the DNA methylation status in the chickens. We also discussed a potential role of 
      variant cBDNF1 in MD tumor resistance and susceptibility.
FAU - Yu, Ying
AU  - Yu Y
AD  - Department of Animal and Avian Sciences, University of Maryland, College Park, MD 
      20742, USA.
FAU - Zhang, Huanmin
AU  - Zhang H
FAU - Byerly, Mardi S
AU  - Byerly MS
FAU - Bacon, Larry D
AU  - Bacon LD
FAU - Porter, Tom E
AU  - Porter TE
FAU - Liu, George E
AU  - Liu GE
FAU - Song, Jiuzhou
AU  - Song J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090306
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Alternative Splicing/*physiology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Animals, Inbred Strains
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Chickens
MH  - CpG Islands/physiology
MH  - DNA Methylation/*physiology
MH  - Female
MH  - Gene Expression Regulation
MH  - Genetic Predisposition to Disease
MH  - Marek Disease/*genetics
MH  - Molecular Sequence Data
MH  - Poultry Diseases/*genetics/*virology
MH  - Promoter Regions, Genetic/physiology
MH  - RNA, Messenger/metabolism
EDAT- 2009/03/10 09:00
MHDA- 2009/07/30 09:00
CRDT- 2009/03/10 09:00
PHST- 2008/07/12 00:00 [received]
PHST- 2008/12/14 00:00 [revised]
PHST- 2009/01/19 00:00 [accepted]
PHST- 2009/03/10 09:00 [entrez]
PHST- 2009/03/10 09:00 [pubmed]
PHST- 2009/07/30 09:00 [medline]
AID - S0006-8993(09)00164-4 [pii]
AID - 10.1016/j.brainres.2009.01.071 [doi]
PST - ppublish
SO  - Brain Res. 2009 May 7;1269:1-10. doi: 10.1016/j.brainres.2009.01.071. Epub 2009 
      Mar 6.