PMID- 19270062
OWN - NLM
STAT- MEDLINE
DCOM- 20090714
LR  - 20211020
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 37
IP  - 9
DP  - 2009 May
TI  - Long 3'-UTRs target wild-type mRNAs for nonsense-mediated mRNA decay in 
      Saccharomyces cerevisiae.
PG  - 2771-8
LID - 10.1093/nar/gkp146 [doi]
AB  - The nonsense-mediated mRNA decay (NMD) pathway, present in most eukaryotic cells, 
      is a specialized pathway that leads to the recognition and rapid degradation of 
      mRNAs with premature termination codons and, importantly, some wild-type mRNAs. 
      Earlier studies demonstrated that aberrant mRNAs with artificially extended 
      3'-untranslated regions (3'-UTRs) are degraded by NMD. However, the extent to 
      which wild-type mRNAs with long 3'-UTRs are degraded by NMD is not known. We used 
      a global approach to identify wild-type mRNAs in Saccharomyces cerevisiae that 
      have longer than expected 3'-UTRs, and of these mRNAs tested, 91% were degraded 
      by NMD. We demonstrate for the first time that replacement of the natural, long 
      3'-UTR from wild-type PGA1 mRNA, which encodes a protein that is important for 
      cell wall biosynthesis, with a short 3'-UTR renders it immune to NMD. The natural 
      PGA1 3'-UTR is sufficient to target a NMD insensitive mRNA for decay by the NMD 
      pathway. Finally, we show that nmd mutants are sensitive to Calcofluor White, 
      which suggests that the regulation of PGA1 and other cell wall biosynthesis 
      proteins by NMD is physiologically significant.
FAU - Kebaara, Bessie W
AU  - Kebaara BW
AD  - School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588, 
      USA.
FAU - Atkin, Audrey L
AU  - Atkin AL
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090306
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Act1 protein, S cerevisiae)
RN  - 0 (Actins)
RN  - 0 (Codon, Nonsense)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.4.1.- (Mannosyltransferases)
RN  - EC 2.4.1.- (PGA1 protein, S cerevisiae)
SB  - IM
MH  - 3' Untranslated Regions/*chemistry
MH  - Actins/genetics
MH  - *Codon, Nonsense
MH  - Gene Expression Regulation, Fungal
MH  - Mannosyltransferases/genetics/metabolism
MH  - Membrane Proteins/genetics/metabolism
MH  - *RNA Stability
MH  - RNA, Messenger/*metabolism
MH  - Saccharomyces cerevisiae/*genetics/metabolism
MH  - Saccharomyces cerevisiae Proteins/genetics/metabolism
PMC - PMC2685090
EDAT- 2009/03/10 09:00
MHDA- 2009/07/15 09:00
PMCR- 2009/03/06
CRDT- 2009/03/10 09:00
PHST- 2009/03/10 09:00 [entrez]
PHST- 2009/03/10 09:00 [pubmed]
PHST- 2009/07/15 09:00 [medline]
PHST- 2009/03/06 00:00 [pmc-release]
AID - gkp146 [pii]
AID - 10.1093/nar/gkp146 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2009 May;37(9):2771-8. doi: 10.1093/nar/gkp146. Epub 2009 Mar 
      6.