PMID- 19273881
OWN - NLM
STAT- MEDLINE
DCOM- 20090615
LR  - 20211020
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 24
IP  - 4
DP  - 2009 Apr
TI  - An in vivo culture system for human embryos using an encapsulation technology: a 
      pilot study.
PG  - 790-6
LID - 10.1093/humrep/dep005 [doi]
AB  - BACKGROUND: Animal studies have demonstrated better embryo development in vivo 
      than in vitro. This pilot study tested the feasibility of using a novel in utero 
      culture system (IUCS) to obtain normal human fertilization and embryo 
      development. METHODS: The IUCS device comprised a perforated silicone hollow 
      tube. The study included 13 patients (<36 years) undergoing a first 
      intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in 
      three groups. In Group 1, 1-2 h after ICSI, sibling oocytes were assigned to IUCS 
      or conventional in vitro culture. The device was retrieved on Day 1, and all 
      zygotes were cultured in vitro till Day 5. In Group 2, fertilized oocytes were 
      assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 
      5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for 
      blastomere biopsy and fluorescence in situ hybridization (FISH) and cultured 
      until Day 5. The highest quality blastocysts were transferred on Day 5. RESULTS: 
      Fertilization and embryo development were comparable in the in vitro and IUCS 
      arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in 
      Group 3 revealed more normal embryos using the IUCS (P = 0.049). Three clinical 
      pregnancies and live births were obtained: two from the IUCS arm and one from the 
      in vitro arm. CONCLUSIONS: Our pilot study shows that this new IUCS appears to be 
      feasible and safe, supporting normal fertilization, embryo development and normal 
      chromosomal segregation. Furthermore, live births are possible after the 
      transient presence of a silicone device in the uterus. Clinicaltrials.gov: 
      NCT00480103.
FAU - Blockeel, C
AU  - Blockeel C
AD  - Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium.
FAU - Mock, P
AU  - Mock P
FAU - Verheyen, G
AU  - Verheyen G
FAU - Bouche, N
AU  - Bouche N
FAU - Le Goff, Ph
AU  - Le Goff P
FAU - Heyman, Y
AU  - Heyman Y
FAU - Wrenzycki, C
AU  - Wrenzycki C
FAU - Hoffmann, K
AU  - Hoffmann K
FAU - Niemann, H
AU  - Niemann H
FAU - Haentjens, P
AU  - Haentjens P
FAU - de Los Santos, M J
AU  - de Los Santos MJ
FAU - Fernandez-Sanchez, M
AU  - Fernandez-Sanchez M
FAU - Velasco, M
AU  - Velasco M
FAU - Aebischer, P
AU  - Aebischer P
FAU - Devroey, P
AU  - Devroey P
FAU - Simon, C
AU  - Simon C
LA  - eng
SI  - ClinicalTrials.gov/NCT00480103
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090309
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Silicones)
SB  - IM
MH  - Embryo Culture Techniques/instrumentation/*methods
MH  - Embryo Transfer
MH  - Embryonic Development
MH  - Equipment Design
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant, Newborn
MH  - Male
MH  - Pilot Projects
MH  - Pregnancy
MH  - Silicones
MH  - Sperm Injections, Intracytoplasmic
MH  - Time Factors
PMC - PMC2656929
EDAT- 2009/03/11 09:00
MHDA- 2009/06/16 09:00
PMCR- 2009/03/10
CRDT- 2009/03/11 09:00
PHST- 2009/03/11 09:00 [entrez]
PHST- 2009/03/11 09:00 [pubmed]
PHST- 2009/06/16 09:00 [medline]
PHST- 2009/03/10 00:00 [pmc-release]
AID - dep005 [pii]
AID - 10.1093/humrep/dep005 [doi]
PST - ppublish
SO  - Hum Reprod. 2009 Apr;24(4):790-6. doi: 10.1093/humrep/dep005. Epub 2009 Mar 9.