PMID- 19273898
OWN - NLM
STAT- MEDLINE
DCOM- 20090511
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 59
IP  - 5
DP  - 2009
TI  - The microorganism community of pentachlorophenol (PCP)-degrading coupled 
      granules.
PG  - 987-94
LID - 10.2166/wst.2009.059 [doi]
AB  - Coupled granules are self-immobilized aggregates of microorganisms under micro 
      aerobic conditions, which have the dissolved oxygen (DO) level of 0.6 mg.L(-1). 
      The effects of DO concentration on pentachlorophenol (PCP) reduction and its 
      microbial community were investigated in a coupled anaerobic and aerobic reactor. 
      Both the diversity and the dynamicity of the Eubacteria and Archaea community, 
      which were responsible for PCP degradation, were evaluated by means of 
      amplification by polymerase chain reaction (PCR) and separation using the 
      denaturing gradient gel electrophoresis (DGGE) technique. The results 
      demonstrated a major shift in the Eubacteria and Archaea community as the mixed 
      aerobic and anaerobic seeding sludge (1:1 by volume) developed into coupled 
      granules and finally acclimated with PCP throughout the experiment period within 
      60 days. The numbers of the Eubacteria population decreased from 20, 16 to 11; 
      Shannon diversity index decreased from 2.75, 2.53 to 2.10. In contrast, the 
      number of the Archaea population increased from 12, 14 to 18; and Shannon 
      diversity index increased from 1.87, 1.88 to 2.43. Phylogenetic analysis based on 
      16SrDNA genes showed the dominance Sphingomonas, Desulfobulbus, Proteobacteria, 
      Actinobacterium, Methanogenic and some uncultured bacteria in the PCP-degrading 
      coupled granules. Microorganism community construction of coupled granules was 
      also deduced.
FAU - Chen, Y C
AU  - Chen YC
AD  - State Key Laboratory of Pulp and Paper Engineering, South China University of 
      Technology, Guangzhou, 510640, Guangdong, China. chenyc@scut.edu.cn
FAU - Chen, D
AU  - Chen D
FAU - Peng, L C
AU  - Peng LC
FAU - Fu, S Y
AU  - Fu SY
FAU - Zhan, H Y
AU  - Zhan HY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA, Bacterial)
RN  - D9BSU0SE4T (Pentachlorophenol)
SB  - IM
MH  - Bioreactors/*microbiology
MH  - DNA, Bacterial/genetics
MH  - Pentachlorophenol/*metabolism
MH  - Phylogeny
EDAT- 2009/03/11 09:00
MHDA- 2009/05/12 09:00
CRDT- 2009/03/11 09:00
PHST- 2009/03/11 09:00 [entrez]
PHST- 2009/03/11 09:00 [pubmed]
PHST- 2009/05/12 09:00 [medline]
AID - 10.2166/wst.2009.059 [doi]
PST - ppublish
SO  - Water Sci Technol. 2009;59(5):987-94. doi: 10.2166/wst.2009.059.