PMID- 19274590
OWN - NLM
STAT- MEDLINE
DCOM- 20100917
LR  - 20100617
IS  - 1520-5762 (Electronic)
IS  - 0363-9045 (Linking)
VI  - 35
IP  - 8
DP  - 2009 Aug
TI  - Tripartite siRNA micelles as controlled delivery systems for primary dendritic 
      cells.
PG  - 950-8
LID - 10.1080/03639040802716653 [doi]
AB  - Dendritic cells (DCs) are key cells in immunology that are able to stimulate or 
      inhibit the immune response. RNA interference has appeared of great interest to 
      modulate the expression of immunogenic or tolerogenic molecules. In our study, 
      pH-sensitive polyion complex micelles based on a double-hydrophilic block 
      copolymer and poly-L-lysine were formulated to entrap a small interfering RNA 
      (siRNA). We show that siRNA-loaded micelles were cytotolerant and efficiently 
      endocytosed by DCs. siRNA targeting eGFP, used as model siRNA, was released into 
      the cytosol following endocytosis of the micelles and the silencing of eGFP 
      expression was observed in DC isolated from transgenic mice. Our results 
      underscore the potential of pH-sensitive polyion complex micelles to formulate 
      therapeutic siRNA for DC engineering in order to maintain the homeostasis of the 
      immune response.
FAU - Boudier, Ariane
AU  - Boudier A
AD  - Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-ENSCM-UM2-UM1, Montpellier, 
      France.
FAU - Aubert-Pouessel, Anne
AU  - Aubert-Pouessel A
FAU - Gerardin, Corine
AU  - Gerardin C
FAU - Devoisselle, Jean-Marie
AU  - Devoisselle JM
FAU - Begu, Sylvie
AU  - Begu S
FAU - Louis-Plence, Pascale
AU  - Louis-Plence P
FAU - Quentin, Julie
AU  - Quentin J
FAU - Jorgensen, Christian
AU  - Jorgensen C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Drug Dev Ind Pharm
JT  - Drug development and industrial pharmacy
JID - 7802620
RN  - 0 (Micelles)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 25104-18-1 (Polylysine)
SB  - IM
MH  - Animals
MH  - Cytosol/metabolism
MH  - Dendritic Cells/*metabolism
MH  - Endocytosis
MH  - *Gene Silencing
MH  - Green Fluorescent Proteins/genetics
MH  - Hydrogen-Ion Concentration
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Micelles
MH  - Polylysine/chemistry
MH  - RNA, Small Interfering/*administration & dosage
EDAT- 2009/03/11 09:00
MHDA- 2010/09/21 06:00
CRDT- 2009/03/11 09:00
PHST- 2009/03/11 09:00 [entrez]
PHST- 2009/03/11 09:00 [pubmed]
PHST- 2010/09/21 06:00 [medline]
AID - 909318127 [pii]
AID - 10.1080/03639040802716653 [doi]
PST - ppublish
SO  - Drug Dev Ind Pharm. 2009 Aug;35(8):950-8. doi: 10.1080/03639040802716653.