PMID- 19274711
OWN - NLM
STAT- MEDLINE
DCOM- 20100319
LR  - 20091223
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 92
IP  - 2
DP  - 2010 Feb
TI  - Heterotypic interaction of fibroblasts and endothelial cells on restricted area.
PG  - 733-45
LID - 10.1002/jbm.a.32364 [doi]
AB  - The polysaccharide hyaluronic acid (Hyal) was photoimmobilized on glass surfaces 
      to obtain a pattern with squares and rectangles of different dimensions and 
      chemistry. The microstructured surfaces were characterized by Scanning Electron 
      Microscopy (SEM), Atomic Force Microscopy (AFM), Attenuated Total Reflection 
      Infrared Spectroscopy (ATR FT-IR), and Time-of-Flight Secondary Ion Mass 
      Spectrometry (ToF-SIMS). Surface analysis revealed the presence of a pattern 
      consisting of alternating glass and Hyal microstructures whose dimensions 
      decreased from the center to the edge of the sample. The behavior of Human 
      Coronary Artery Endothelial Cells (HCAEC) and human tumoral dermal fibroblasts 
      (C54) was studied on these micropatterned surfaces. Neither HCAEC nor C54 adhered 
      to the immobilized Hyal but both adapted their shape to the different sizes of 
      the glass squares and rectangles. The number of adherent HCAEC and C54 depended 
      on the dimensions of both the glass domains and the nuclei of the cells. 
      Co-cultured C54 on HCAEC patterned surfaces showed a heterotypic cell-cell 
      interaction in the same chemical and topographic domain for the first time. In 
      comparison to other techniques for patterning two different cell types, our 
      approach was non cytotoxic and allowed arbitrary geometric patterns to form on 
      different biocompatible substrata.
CI  - (c) 2009 Wiley Periodicals, Inc.
FAU - Lamponi, S
AU  - Lamponi S
AD  - Department of Chemical and Biosystems Science and Technologies and CRISMA, 
      University of Siena, Via A. Moro, 2, Siena 53100, Italy.
FAU - Di Canio, C
AU  - Di Canio C
FAU - Forbicioni, M
AU  - Forbicioni M
FAU - Barbucci, R
AU  - Barbucci R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Count
MH  - Cell Line
MH  - Cell Nucleus/ultrastructure
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytoplasm/ultrastructure
MH  - Endothelial Cells/*physiology/ultrastructure
MH  - Fibroblasts/*physiology/ultrastructure
MH  - Glass
MH  - Humans
MH  - Hyaluronic Acid/chemistry
MH  - Mass Spectrometry
MH  - Microscopy, Atomic Force
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Video
MH  - Spectrophotometry, Infrared
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2009/03/11 09:00
MHDA- 2010/03/20 06:00
CRDT- 2009/03/11 09:00
PHST- 2009/03/11 09:00 [entrez]
PHST- 2009/03/11 09:00 [pubmed]
PHST- 2010/03/20 06:00 [medline]
AID - 10.1002/jbm.a.32364 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2010 Feb;92(2):733-45. doi: 10.1002/jbm.a.32364.