PMID- 19274715
OWN - NLM
STAT- MEDLINE
DCOM- 20100319
LR  - 20220512
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 92
IP  - 2
DP  - 2010 Feb
TI  - Comparative characterization of cultures of primary human macrophages or 
      dendritic cells relevant to biomaterial studies.
PG  - 791-800
LID - 10.1002/jbm.a.32406 [doi]
AB  - Macrophages are central mediators of biomaterial-associated wound healing. 
      Dendritic cells (DCs) link innate and adaptive immunity and are important in the 
      context of the host response to combination products. Starting with human 
      peripheral blood mononuclear cells (PBMCs), DCs were derived from monocytes upon 
      culture with granulocyte macrophage colony-stimulating factor and interleukin-4; 
      macrophages were derived from monocytes upon culture without cytokines. 
      Macrophage or DC cultures were characterized at relevant timepoints in both 
      adherent and nonadherent fractions on control Primaria surfaces to characterize 
      and define these inflammatory/immune cells as a prequel to their use in in vitro 
      test biomaterial-host response studies. At day 10 (typical time for harvesting 
      macrophages for subsequent treatment with test biomaterials), macrophages were 
      CD11c+, macrophage mannose receptor (MMR)+, CD14+, and CD64+. At day 6 (typical 
      time for harvesting of DCs after 24-h treatment with test biomaterials), DCs were 
      CD1c+, CD11c+, CD123+, MMR+, CD14+, and CD64-. Furthermore, CD3+ and CD4+ T 
      lymphocytes and CD19+ and CD24+ B lymphocytes were present in both cultures at 
      all timepoints, although to different extents. Immature DCs (approximately 15 
      microm), were rounded but presented extensive dendritic processes upon maturation 
      with lipopolysaccharide. Alternatively, adherent macrophages (approximately 15-20 
      microm) displayed internalized lipids and exhibited few membrane processes. The 
      characterization and comparison of existing techniques to establish reliable, 
      reproducible primary cultures of DCs or macrophages provides an important basis 
      for examining and interpreting complex macrophage/DC-lymphocyte-orchestrated host 
      responses in future studies with equivalent cell populations on test 
      biomaterials.
CI  - (c) 2009 Wiley Periodicals, Inc.
FAU - Shankar, Sucharita P
AU  - Shankar SP
AD  - Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of 
      Technology and Emory University, 313 Ferst Drive Atlanta, Georgia 30332, USA.
FAU - Babensee, Julia E
AU  - Babensee JE
LA  - eng
GR  - R01 EB004633/EB/NIBIB NIH HHS/United States
GR  - 1R01 EB004633-01A1/EB/NIBIB NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Biocompatible Materials)
RN  - 0 (Biomarkers)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/drug effects
MH  - Biocompatible Materials/*pharmacology
MH  - Biomarkers/metabolism
MH  - CD4-Positive T-Lymphocytes
MH  - Cell Adhesion
MH  - Cells, Cultured
MH  - Dendritic Cells/*physiology
MH  - Flow Cytometry
MH  - Granulocyte Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-4/pharmacology
MH  - Macrophages/*physiology
MH  - Materials Testing/*methods
MH  - Swine
EDAT- 2009/03/11 09:00
MHDA- 2010/03/20 06:00
CRDT- 2009/03/11 09:00
PHST- 2009/03/11 09:00 [entrez]
PHST- 2009/03/11 09:00 [pubmed]
PHST- 2010/03/20 06:00 [medline]
AID - 10.1002/jbm.a.32406 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2010 Feb;92(2):791-800. doi: 10.1002/jbm.a.32406.