PMID- 19274716
OWN - NLM
STAT- MEDLINE
DCOM- 20100319
LR  - 20161125
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 92
IP  - 2
DP  - 2010 Feb
TI  - Incorporation of collagen in poly(3,4-ethylenedioxythiophene) for a bifunctional 
      film with high bio- and electrochemical activity.
PG  - 766-72
LID - 10.1002/jbm.a.32412 [doi]
AB  - Electrochemical polymerization can be used to directly synthesize conducting 
      polymers while incorporating different functional molecules such as proteins for 
      specific applications. There is a need to systematically study the effects of 
      synthetic conditions for a polymer/protein composite on its nanostructure, 
      chem/physical properties, and bioactivities. In this study, collagen, a 
      cell-adhesion protein, was impregnated in poly(3,4-ethylenedioxythiophene) 
      (PEDOT) via galvanostatic electropolymerization, and demonstrated binding with 
      the PEDOT backbone and excellent stability. The polymer polymerized at lower 
      current densities shows good electroactivity. Oxidation level of PEDOT was 
      investigated by Raman spectroscopy. The results show that simple inorganic anions 
      are more readily to be doped into the polymer than the bulky collagen molecules. 
      Scanning electron microscope (SEM) observation reveals that the PEDOT/collagen 
      system has a surface morphology different from those previously studied, 
      exhibiting a network structure with nano-silks interlacing. Optical microscopy 
      examination shows that rat pheochromocytoma (PC12) cells are preferentially 
      seeded on PEDOT/collagen other than PEDOT/LiClO(4), indicating that collagen is 
      highly bioactive for cell adhesion. Further study shows that synthesis with a 
      lower current density favors the incorporation of collagen and thus increases the 
      cell attachment to the PEDOT/collagen matrix. This study renders a simple 
      approach to tailor a bifunctional film with high bio- and electrochemical 
      activity.
CI  - (c) 2009 Wiley Periodicals, Inc.
FAU - Xiao, Yinghong
AU  - Xiao Y
AD  - School of Chemical and Biomedical Engineering, Nanyang Technological University, 
      Singapore.
FAU - Li, Chang Ming
AU  - Li CM
FAU - Wang, Shenqi
AU  - Wang S
FAU - Shi, Jingsheng
AU  - Shi J
FAU - Ooi, Chiu Ping
AU  - Ooi CP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Polymers)
RN  - 0 (poly(3,4-ethylene dioxythiophene))
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*chemistry
MH  - Bridged Bicyclo Compounds, Heterocyclic/*chemistry
MH  - Collagen/*chemistry
MH  - Electrochemistry
MH  - Electrodes
MH  - *Membranes, Artificial
MH  - Microscopy, Electron, Scanning
MH  - PC12 Cells
MH  - Polymers/*chemistry
MH  - Potentiometry
MH  - Rats
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Spectrum Analysis, Raman
EDAT- 2009/03/11 09:00
MHDA- 2010/03/20 06:00
CRDT- 2009/03/11 09:00
PHST- 2009/03/11 09:00 [entrez]
PHST- 2009/03/11 09:00 [pubmed]
PHST- 2010/03/20 06:00 [medline]
AID - 10.1002/jbm.a.32412 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2010 Feb;92(2):766-72. doi: 10.1002/jbm.a.32412.