PMID- 19276534
OWN - NLM
STAT- MEDLINE
DCOM- 20090508
LR  - 20191027
IS  - 0951-6433 (Print)
IS  - 0951-6433 (Linking)
VI  - 33
IP  - 1
DP  - 2008
TI  - Auraptene, a citrus fruit compound, regulates gene expression as a PPARalpha 
      agonist in HepG2 hepatocytes.
PG  - 25-32
AB  - Citrus fruit compounds have various activities that improve pathological 
      conditions in many tissues. In this study, we examined the effect of auraptene 
      contained mainly in the peel of citrus on peroxisome proliferator-activated 
      receptor-alpha (PPARalpha) activation. To examine effects of auraptene on the 
      PPARalpha activation in hepatocytes, PPAR ligand assay system was developed using 
      HepG2 hepatocytes, in which the endogenous PPARalpha expression level is very 
      low. In the PPAR ligand assay, the addition of auraptene showed significant 
      effects on the transactivation of GAL4/PPARalpha chimera proteins in a 
      dose-dependent manner. Actually, treatment with auraptene induced the 
      up-regulation of PPAR target genes, such as acyl-CoA oxidase (ACO), 
      carnitine-palmitoyl transferase 1A (CPT1A) and acyl-CoA synthetase (ACS), in 
      PPARalpha-expressing HepG2 hepatocytes. The regulation of gene expression was 
      dependent on PPARalpha because mock-transfected HepG2 hepatocytes showed no 
      regulation. The up-regulation of PPAR target gene expression by auraptene was 
      sufficient to enhance oleic acid uptake into PPARalpha-expressing HepG2 
      hepatocytes. These results indicate that auraptene acts as a PPARalpha agonist in 
      hepatocytes and that auraptene may improve lipid abnormality through PPARalpha 
      activation in the liver.
FAU - Takahashi, Nobuyuki
AU  - Takahashi N
AD  - Laboratory of Molecular Function of Food, Division of Food Science and 
      Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 
      Japan.
FAU - Kang, Min-Sook
AU  - Kang MS
FAU - Kuroyanagi, Kayo
AU  - Kuroyanagi K
FAU - Goto, Tsuyoshi
AU  - Goto T
FAU - Hirai, Shizuka
AU  - Hirai S
FAU - Ohyama, Kana
AU  - Ohyama K
FAU - Lee, Joo-Young
AU  - Lee JY
FAU - Yu, Rina
AU  - Yu R
FAU - Yano, Masamichi
AU  - Yano M
FAU - Sasaki, Takao
AU  - Sasaki T
FAU - Murakami, Shigeru
AU  - Murakami S
FAU - Kawada, Teruo
AU  - Kawada T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Coumarins)
RN  - 0 (Fatty Acids)
RN  - 0 (PPAR alpha)
RN  - F79I1ZEL2E (aurapten)
SB  - IM
MH  - Cell Line, Tumor
MH  - Coumarins/*pharmacology
MH  - Fatty Acids/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Hepatocytes/drug effects
MH  - Humans
MH  - PPAR alpha/*agonists/biosynthesis
EDAT- 2008/01/01 00:00
MHDA- 2009/05/09 09:00
CRDT- 2009/03/12 09:00
PHST- 2009/03/12 09:00 [entrez]
PHST- 2008/01/01 00:00 [pubmed]
PHST- 2009/05/09 09:00 [medline]
AID - 80435472L815VR2U [pii]
AID - 10.1002/biof.5520330103 [doi]
PST - ppublish
SO  - Biofactors. 2008;33(1):25-32. doi: 10.1002/biof.5520330103.