PMID- 19277010
OWN - NLM
STAT- MEDLINE
DCOM- 20090629
LR  - 20211020
IS  - 1525-0024 (Electronic)
IS  - 1525-0016 (Print)
IS  - 1525-0016 (Linking)
VI  - 17
IP  - 5
DP  - 2009 May
TI  - Baculovirus transduction of mesenchymal stem cells: in vitro responses and in 
      vivo immune responses after cell transplantation.
PG  - 889-96
LID - 10.1038/mt.2009.30 [doi]
AB  - Baculovirus holds great promise for the genetic modification of mesenchymal stem 
      cells (MSCs). However, whether baculovirus transduction provokes undesired MSCs 
      responses that might compromise their in vivo applications has yet to be 
      examined. Hereby, we unraveled that baculovirus transduction of human MSCs 
      upregulated the transcription of interleukin (IL)-1beta, interferon (IFN)-alpha 
      and IL-6, but not tumor necrosis factor (TNF)-alpha and IFN-gamma. However, only 
      IL-6 secretion was detectable by enzyme-linked immunosorbent assay (ELISA). 
      Baculovirus transduction also stimulated transient, low level upregulation of 
      human leukocyte antigen I (HLA-I) on the human MSCs surface, yet it did not 
      either altered the HLA-II expression or impaired the MSCs ability to inhibit 
      lymphocyte proliferation. After transplantation into allogeneic rats, the 
      transduced rat MSCs elicited transient, mild macrophage responses, but the cells 
      remained tolerant as judged by the persistence of transplanted cells and absence 
      of CD8(+) T cells infiltration. Besides, transplantation of the transduced MSCs 
      did not provoke systemic induction of monocytes and CD8(+) T cells. This study, 
      for the first time, explores the responses of MSCs to virus transduction and 
      confirms the safety of transplanting baculovirus-engineered MSCs into 
      immunocompetent animals for cell-based gene therapy.
FAU - Chuang, Ching-Kuang
AU  - Chuang CK
AD  - Department of Chemical Engineering, National Tsing Hua University, Hsinchu, 
      Taiwan.
FAU - Wong, Tong-Hong
AU  - Wong TH
FAU - Hwang, Shiaw-Min
AU  - Hwang SM
FAU - Chang, Yu-Han
AU  - Chang YH
FAU - Chen, Guan-Yu
AU  - Chen GY
FAU - Chiu, Yung-Chung
AU  - Chiu YC
FAU - Huang, Shiu-Feng
AU  - Huang SF
FAU - Hu, Yu-Chen
AU  - Hu YC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090310
PL  - United States
TA  - Mol Ther
JT  - Molecular therapy : the journal of the American Society of Gene Therapy
JID - 100890581
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Baculoviridae/*genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Interferon-alpha/metabolism
MH  - Interferon-gamma/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*immunology/*virology
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Transduction, Genetic
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC2835125
EDAT- 2009/03/12 09:00
MHDA- 2009/06/30 09:00
PMCR- 2010/05/01
CRDT- 2009/03/12 09:00
PHST- 2009/03/12 09:00 [entrez]
PHST- 2009/03/12 09:00 [pubmed]
PHST- 2009/06/30 09:00 [medline]
PHST- 2010/05/01 00:00 [pmc-release]
AID - S1525-0016(16)31789-0 [pii]
AID - 10.1038/mt.2009.30 [doi]
PST - ppublish
SO  - Mol Ther. 2009 May;17(5):889-96. doi: 10.1038/mt.2009.30. Epub 2009 Mar 10.