PMID- 19277578
OWN - NLM
STAT- MEDLINE
DCOM- 20090519
LR  - 20090409
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 538
DP  - 2009
TI  - Fluorescence In situ hybridization (FISH) as a tool for the detection of 
      significant chromosomal abnormalities in childhood leukaemia.
PG  - 29-55
LID - 10.1007/978-1-59745-418-6_3 [doi]
AB  - Cytogenetics is integral to the diagnosis of childhood leukaemia, particularly in 
      relation to the risk stratification of patients for treatment. Fluorescence in 
      situ hybridization (FISH) has become an important complementary technique, 
      expanding chromosomal analysis into the molecular arena. It has greatly improved 
      the accuracy and applicability of cytogenetics and led to the discovery of novel 
      chromosomal changes of prognostic significance. Many probes are now commercially 
      available, providing robust and reliable detection of chromosomal abnormalities. 
      Since the cloning of the human genome, it is now possible to access detailed 
      genomic information and develop FISH probes for virtually any known DNA sequence. 
      The range of procedures necessary for the successful application of FISH in the 
      accurate detection of significant chromosomal abnormalities in childhood acute 
      leukaemia is described here.
FAU - Konn, Zoe J
AU  - Konn ZJ
AD  - Cancer Sciences Division, University of Southampton, Southampton, UK.
FAU - Wright, Sarah L
AU  - Wright SL
FAU - Barber, Kerry E
AU  - Barber KE
FAU - Harrison, Christine J
AU  - Harrison CJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Probes)
SB  - IM
MH  - Bone Marrow/pathology
MH  - *Chromosome Aberrations
MH  - DNA Probes/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Hybridization
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 2009/03/12 09:00
MHDA- 2009/05/20 09:00
CRDT- 2009/03/12 09:00
PHST- 2009/03/12 09:00 [entrez]
PHST- 2009/03/12 09:00 [pubmed]
PHST- 2009/05/20 09:00 [medline]
AID - 10.1007/978-1-59745-418-6_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2009;538:29-55. doi: 10.1007/978-1-59745-418-6_3.