PMID- 19279664
OWN - NLM
STAT- MEDLINE
DCOM- 20090504
LR  - 20211020
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 28
IP  - 8
DP  - 2009 Apr 22
TI  - Spt6 enhances the elongation rate of RNA polymerase II in vivo.
PG  - 1067-77
LID - 10.1038/emboj.2009.56 [doi]
AB  - Several eukaryotic transcription factors have been shown to modulate the 
      elongation rate of RNA polymerase II (Pol II) on naked or chromatin-reconstituted 
      templates in vitro. However, none of the tested factors have been shown to 
      directly affect the elongation rate of Pol II in vivo. We performed a directed 
      RNAi knock-down (KD) screen targeting 141 candidate transcription factors and 
      identified multiple factors, including Spt6, that alter the induced Hsp70 
      transcript levels in Drosophila S2 cells. Spt6 is known to interact with both 
      nucleosome structure and Pol II, and it has properties consistent with having a 
      role in elongation. Here, ChIP assays of the first wave of Pol II after heat 
      shock in S2 cells show that KD of Spt6 reduces the rate of Pol II elongation. 
      Also, fluorescence recovery after photobleaching assays of GFP-Pol II in salivary 
      gland cells show that this Spt6-dependent effect on elongation rate persists 
      during steady-state-induced transcription, reducing the elongation rate from 
      approximately 1100 to 500 bp/min. Furthermore, RNAi depletion of Spt6 reveals its 
      broad requirement during different stages of development.
FAU - Ardehali, M Behfar
AU  - Ardehali MB
AD  - Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 
      14853, USA.
FAU - Yao, Jie
AU  - Yao J
FAU - Adelman, Karen
AU  - Adelman K
FAU - Fuda, Nicholas J
AU  - Fuda NJ
FAU - Petesch, Steven J
AU  - Petesch SJ
FAU - Webb, Watt W
AU  - Webb WW
FAU - Lis, John T
AU  - Lis JT
LA  - eng
GR  - R01 GM025232/GM/NIGMS NIH HHS/United States
GR  - R01 GM025232-33/GM/NIGMS NIH HHS/United States
GR  - R37 GM025232/GM/NIGMS NIH HHS/United States
GR  - GM25232/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090312
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Drosophila Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Histones)
RN  - 0 (Hsp83 protein, Drosophila)
RN  - 0 (Peptide Elongation Factors)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Spt6 protein, Drosophila)
RN  - 0 (Transcriptional Elongation Factors)
RN  - 0 (atms protein, Drosophila)
RN  - 0 (transcription factor S-II)
RN  - 138673-72-0 (SPT5 transcriptional elongation factor)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Cell Line
MH  - Chromosomal Proteins, Non-Histone/genetics/metabolism
MH  - Drosophila Proteins/genetics/*metabolism
MH  - Drosophila melanogaster/genetics/*physiology
MH  - Fluorescence Recovery After Photobleaching
MH  - HSP70 Heat-Shock Proteins/genetics/metabolism
MH  - Heat-Shock Proteins/genetics/metabolism
MH  - Histones/metabolism
MH  - Peptide Elongation Factors/genetics/*metabolism
MH  - RNA Interference
MH  - RNA Polymerase II/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Transcription, Genetic
MH  - Transcriptional Elongation Factors/genetics/metabolism
PMC - PMC2683705
EDAT- 2009/03/13 09:00
MHDA- 2009/05/05 09:00
PMCR- 2010/04/22
CRDT- 2009/03/13 09:00
PHST- 2008/10/30 00:00 [received]
PHST- 2009/02/06 00:00 [accepted]
PHST- 2009/03/13 09:00 [entrez]
PHST- 2009/03/13 09:00 [pubmed]
PHST- 2009/05/05 09:00 [medline]
PHST- 2010/04/22 00:00 [pmc-release]
AID - emboj200956 [pii]
AID - 10.1038/emboj.2009.56 [doi]
PST - ppublish
SO  - EMBO J. 2009 Apr 22;28(8):1067-77. doi: 10.1038/emboj.2009.56. Epub 2009 Mar 12.