PMID- 19283847
OWN - NLM
STAT- MEDLINE
DCOM- 20091022
LR  - 20091119
IS  - 1097-0347 (Electronic)
IS  - 1043-3074 (Linking)
VI  - 31
IP  - 7
DP  - 2009 Jul
TI  - Receptor tyrosine kinases in sinonasal undifferentiated carcinomas--evaluation 
      for EGFR, c-KIT, and HER2/neu expression.
PG  - 919-27
LID - 10.1002/hed.21061 [doi]
AB  - BACKGROUND: Our objective was to identify the expression of epidermal growth 
      factor receptor (EGFR), c-KIT (CD117), and HER2/neu in sinonasal undifferentiated 
      carcinoma (SNUC). METHODS: Immunohistochemistry for c-KIT (CD117), EGFR, and 
      HER2/neu was performed on paraffin-embedded tissue from SNUC cases. A search for 
      activating mutations in c-kit exons 9, 11, 13, and 17 or gene amplification was 
      undertaken by high-resolution DNA melting curve analysis and fluorescence in situ 
      hybridization (FISH) for c-kit and chromosome 4, respectively. RESULTS: By 
      immunohistochemistry, 9 of 11 cases (81.8%) were diffusely (4+) positive for 
      c-KIT, 3 of 11 cases (27.3%) were positive for EGFR, and none of the cases were 
      positive for HER2/neu. Neither activating mutations nor gene amplification of 
      c-kit were detected in any of the 8 assessable tumors. CONCLUSION: c-KIT is 
      frequently expressed in SNUC. However, the overexpression is not due to 
      activating mutations or gene amplification.
FAU - Chernock, Rebecca D
AU  - Chernock RD
AD  - Department of Pathology and Immunology, Washington University School of Medicine, 
      St. Louis, Missouri, USA.
FAU - Perry, Arie
AU  - Perry A
FAU - Pfeifer, John D
AU  - Pfeifer JD
FAU - Holden, Joseph A
AU  - Holden JA
FAU - Lewis, James S Jr
AU  - Lewis JS Jr
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma/*enzymology/*genetics/pathology
MH  - Cohort Studies
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mutation/*genetics
MH  - Paranasal Sinus Neoplasms/*enzymology/*genetics/pathology
MH  - Polymerase Chain Reaction
MH  - Receptor Protein-Tyrosine Kinases/*physiology
MH  - Retrospective Studies
EDAT- 2009/03/14 09:00
MHDA- 2009/10/23 06:00
CRDT- 2009/03/14 09:00
PHST- 2009/03/14 09:00 [entrez]
PHST- 2009/03/14 09:00 [pubmed]
PHST- 2009/10/23 06:00 [medline]
AID - 10.1002/hed.21061 [doi]
PST - ppublish
SO  - Head Neck. 2009 Jul;31(7):919-27. doi: 10.1002/hed.21061.