PMID- 19284957
OWN - NLM
STAT- MEDLINE
DCOM- 20140123
LR  - 20190923
IS  - 1672-1977 (Print)
IS  - 1672-1977 (Linking)
VI  - 7
IP  - 3
DP  - 2009 Mar
TI  - [Effects of Pingchuan Recipe on airway remodeling in mice with bronchial asthma].
PG  - 261-7
AB  - OBJECTIVE: To study the effects of Pingchuan Recipe, a compound traditional 
      Chinese herbal medicine for treating bronchial asthma, on macrophage inflammatory 
      protein-1alpha (MIP-1alpha) and immunoglobulin E (IgE) contents and CD86 
      expression in a mouse model of bronchial asthma, and to investigate the mechanism 
      of Pingchuan Recipe in regulating airway remodeling in mice with bronchial 
      asthma. METHODS: Fifty BALB/c mice were randomly divided into normal control 
      group, untreated group, dexamethasone group, low-dose Pingchuan Recipe group and 
      normal-dose Pingchuan Recipe group. Bronchial asthma in mice was induced by 
      intra-abdominal injection of mixed ovalbumin and stimulation by inhaling 5% 
      ovalbumin. Mice in the normal control group and the untreated group did not 
      receive treatment, but mice in the other groups were administered 
      intragastrically with 50 g/ (kg * d) Pingchuan Recipe, 25 g/ (kg * d) Pingchuan 
      Recipe and 0.75 mg/ (kg * d) dexamethasone, respectively. After consecutive 
      treatment for 8 weeks, the MIP-1alpha contents in peripheral blood and 
      bronchoalveolar lavage fluid (BALF) were measured by an avidin biotin 
      complex-enzyme-linked immunosorbent assay (ABC-ELISA) system, and the IgE 
      contents in serum and BALF were measured by ELISA method. Direct 
      immunofluorescence-flow cytometry was used to detect the percentages of CD86(+) 
      and CD3(-) CD86(+) cells in peripheral blood and BALF. General histological 
      changes of the lung tissue were observed by HE staining and collagen fiber 
      staining 12 weeks after activation. RESULTS: Compared with the normal control 
      group, the contents of MIP-1alpha, the percentages of CD86(+) cells and CD3(-) 
      CD86(+) cells in peripheral blood and BALF, and the contents of IgE in serum and 
      BALF were raised significantly (P < 0.01). The contents of MIP-1alpha, the 
      percentages of CD86(+) cells and CD3(-) CD86(+) cells in peripheral blood and 
      BALF, and the contents of IgE in serum and BALF in the treated groups were lower 
      than those in the untreated group (P < 0.01). The contents of MIP-1alpha in 
      peripheral blood and BALF and the contents of IgE in serum and BALF had no 
      significant differences among the treated groups (P > 0.05). The percentage of 
      CD3(-) CD86(+) cells in peripheral blood in low-dose Pingchuan Recipe group was 
      lower than that in the dexamethasone group. Compared with the untreated group, 
      the collagen deposition in the wall of bronchiole in the treated groups was 
      decreased. CONCLUSION: Pingchuan Recipe can decrease the contents of MIP-1alpha, 
      IgE and the expressions of CD86 in peripheral blood and BALF, which may be one of 
      its mechanisms in improving chronic airway inflammation and inhibiting airway 
      remodeling.
FAU - Zhu, Hui-hua
AU  - Zhu HH
AD  - Department of Pediatrics, Yueyang Hospital of Integrated Traditional Chinese and 
      Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 
      200437, China.
FAU - Yu, Jian-er
AU  - Yu JE
FAU - Zhang, Xiao-feng
AU  - Zhang XF
FAU - Chen, Yan-ping
AU  - Chen YP
FAU - Pang, Hui-fang
AU  - Pang HF
FAU - Wang, Guo-hua
AU  - Wang GH
FAU - Guan, Yu
AU  - Guan Y
FAU - Wu, Jie
AU  - Wu J
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhong Xi Yi Jie He Xue Bao
JT  - Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
JID - 101199657
RN  - 0 (B7-2 Antigen)
RN  - 0 (Ccl3 protein, mouse)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Airway Remodeling
MH  - Animals
MH  - Asthma/drug therapy/*metabolism/*physiopathology
MH  - B7-2 Antigen/metabolism
MH  - Bronchoalveolar Lavage Fluid
MH  - Chemokine CCL3/metabolism
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*pharmacology/therapeutic use
MH  - Immunoglobulin E/metabolism
MH  - Inflammation/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 2009/03/17 09:00
MHDA- 2014/01/24 06:00
CRDT- 2009/03/17 09:00
PHST- 2009/03/17 09:00 [entrez]
PHST- 2009/03/17 09:00 [pubmed]
PHST- 2014/01/24 06:00 [medline]
AID - jcim20090312 [pii]
AID - 10.3736/jcim20090312 [doi]
PST - ppublish
SO  - Zhong Xi Yi Jie He Xue Bao. 2009 Mar;7(3):261-7. doi: 10.3736/jcim20090312.