PMID- 19285014
OWN - NLM
STAT- MEDLINE
DCOM- 20090420
LR  - 20211203
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 380
IP  - 3
DP  - 2009 Mar 13
TI  - The serine 2481-autophosphorylated form of mammalian Target Of Rapamycin (mTOR) 
      is localized to midzone and midbody in dividing cancer cells.
PG  - 638-43
LID - 10.1016/j.bbrc.2009.01.153 [doi]
AB  - Using a high-resolution, automated confocal high-content imaging system, we 
      investigated the sub-cellular localization of the Serine 2481-autophosphorylated 
      form of mTOR (PP-mTOR(Ser2481)) during mitosis and cytokinesis in human cancer 
      cells. PP-mTOR(Ser2481) exhibited a punctate nuclear distribution in interphase 
      cancer cells, with the number of PP-mTOR(Ser2481) nuclear speckles positively 
      relating with the proliferative capacity of cancer cells. PP-mTOR(Ser2481) 
      expression dynamically rearranged within the cytoplasm in a close association 
      near and between separating chromosomes during early stages of mitosis. Towards 
      the end of anaphase and in telophase, PP-mTOR(Ser2481) drastically focused on the 
      midzone and ultimately in the centre of the midbody at the presumptive cleavage 
      furrow. In cells at cytokinesis, PP-mTOR(Ser2481) appeared as a doublet facing 
      each other at the apical ends of two daughter cells. Three-dimensional analysis 
      confirmed that PP-mTOR(Ser2481) positioned at a ring structure wrapped round by 
      microtubule bundles to connect daughter cells. These results reveal for the first 
      time that PP-mTOR(Ser2481) may be unexpectedly involved in the terminal stages of 
      cytokinesis.
FAU - Vazquez-Martin, Alejandro
AU  - Vazquez-Martin A
AD  - Catalan Institute of Oncology (ICO Girona), Dr. Josep Trueta University Hospital 
      of Girona, Avenida de Francia s/n, E-17007 Girona, Catalonia, Spain.
FAU - Oliveras-Ferraros, Cristina
AU  - Oliveras-Ferraros C
FAU - Bernado, Luis
AU  - Bernado L
FAU - Lopez-Bonet, Eugeni
AU  - Lopez-Bonet E
FAU - Menendez, Javier A
AU  - Menendez JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090129
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Carrier Proteins)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Carrier Proteins/*metabolism
MH  - Cell Division
MH  - Cell Line, Tumor
MH  - Cell Nucleus/enzymology
MH  - Cell Proliferation
MH  - Humans
MH  - Neoplasms/*metabolism/*pathology
MH  - Phosphorylation
MH  - Phosphotransferases (Alcohol Group Acceptor)/*metabolism
MH  - Serine/*metabolism
MH  - TOR Serine-Threonine Kinases
EDAT- 2009/03/17 09:00
MHDA- 2009/04/21 09:00
CRDT- 2009/03/17 09:00
PHST- 2009/01/19 00:00 [received]
PHST- 2009/01/23 00:00 [accepted]
PHST- 2009/03/17 09:00 [entrez]
PHST- 2009/03/17 09:00 [pubmed]
PHST- 2009/04/21 09:00 [medline]
AID - S0006-291X(09)00195-8 [pii]
AID - 10.1016/j.bbrc.2009.01.153 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Mar 13;380(3):638-43. doi: 
      10.1016/j.bbrc.2009.01.153. Epub 2009 Jan 29.