PMID- 19285653
OWN - NLM
STAT- MEDLINE
DCOM- 20090518
LR  - 20091119
IS  - 1097-6868 (Electronic)
IS  - 0002-9378 (Linking)
VI  - 200
IP  - 5
DP  - 2009 May
TI  - Persistence of male hematopoietic CD34+ cells in the circulation of women does 
      not affect prenatal diagnostic techniques.
PG  - 528.e1-7
LID - 10.1016/j.ajog.2008.12.019 [doi]
AB  - OBJECTIVE: We aimed to verify whether fetal microchimerism, because of persisting 
      fetal hematopoietic CD34(+) cells from previous pregnancies, could interfere with 
      the development of genetic tests based on using these cells, isolated from 
      maternal blood for the diagnosis of fetal aneuploidies. STUDY DESIGN: CD34(+) 
      cells, isolated from blood of parous women with at least 1 son and nulliparous 
      women, were analyzed by using qualitative polymerase chain reaction (PCR), 
      quantitative PCR, and fluorescence in situ hybridization (FISH) to establish 
      whether these molecular techniques are concurrently capable of detecting 
      circulating male DNA. RESULTS: By qualitative PCR, male DNA was found both in 
      parous and nulliparous women, whereas by quantitative PCR and FISH analyses, no 
      male DNA or male nuclei were revealed except in 1 cultured CD34(+) sample from a 
      nulliparous woman. CONCLUSION: Fetal hematopoietic CD34(+) cells can be used in 
      the noninvasive prenatal testing of fetal aneuploidies because the presence of 
      fetal microchimerism does not affect fetal diagnosis in current pregnancies.
FAU - Coata, Giuliana
AU  - Coata G
AD  - Department of Obstetrics and Gynecology, University Hospital, Perugia, Italy. 
      cmpr-lab@unipg.it
FAU - Picchiassi, Elena
AU  - Picchiassi E
FAU - Centra, Michela
AU  - Centra M
FAU - Fanetti, Alessia
AU  - Fanetti A
FAU - Maula, Vincenza
AU  - Maula V
FAU - Benedetto, Chiara
AU  - Benedetto C
FAU - Di Renzo, Gian Carlo
AU  - Di Renzo GC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090314
PL  - United States
TA  - Am J Obstet Gynecol
JT  - American journal of obstetrics and gynecology
JID - 0370476
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, CD34/metabolism
MH  - *Chimerism
MH  - Chromosome Disorders/diagnosis/genetics
MH  - Female
MH  - Fetal Diseases/*diagnosis/*genetics
MH  - Genetic Testing
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Pregnancy
MH  - *Prenatal Diagnosis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Young Adult
EDAT- 2009/03/17 09:00
MHDA- 2009/05/19 09:00
CRDT- 2009/03/17 09:00
PHST- 2008/05/27 00:00 [received]
PHST- 2008/08/07 00:00 [revised]
PHST- 2008/12/05 00:00 [accepted]
PHST- 2009/03/17 09:00 [entrez]
PHST- 2009/03/17 09:00 [pubmed]
PHST- 2009/05/19 09:00 [medline]
AID - S0002-9378(08)02402-2 [pii]
AID - 10.1016/j.ajog.2008.12.019 [doi]
PST - ppublish
SO  - Am J Obstet Gynecol. 2009 May;200(5):528.e1-7. doi: 10.1016/j.ajog.2008.12.019. 
      Epub 2009 Mar 14.